Protein Info for SO2626 in Shewanella oneidensis MR-1

Name: clpA
Annotation: ATP-dependent Clp protease, ATP-binding subunit ClpA (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 755 TIGR02639: ATP-dependent Clp protease ATP-binding subunit ClpA" amino acids 3 to 735 (733 residues), 1087.6 bits, see alignment E=0 PF02861: Clp_N" amino acids 13 to 62 (50 residues), 51.8 bits, see alignment 4.7e-17 PF00004: AAA" amino acids 212 to 317 (106 residues), 47.3 bits, see alignment E=1.9e-15 amino acids 493 to 608 (116 residues), 39.8 bits, see alignment E=4.1e-13 PF17871: AAA_lid_9" amino acids 351 to 453 (103 residues), 91.6 bits, see alignment E=1.8e-29 PF07724: AAA_2" amino acids 487 to 648 (162 residues), 202.3 bits, see alignment E=3.5e-63 PF00158: Sigma54_activat" amino acids 491 to 616 (126 residues), 23 bits, see alignment E=3.6e-08 PF07728: AAA_5" amino acids 492 to 608 (117 residues), 44.1 bits, see alignment E=1.3e-14 PF10431: ClpB_D2-small" amino acids 654 to 733 (80 residues), 80.6 bits, see alignment E=4.7e-26

Best Hits

Swiss-Prot: 70% identical to CLPA_ECOL6: ATP-dependent Clp protease ATP-binding subunit ClpA (clpA) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K03694, ATP-dependent Clp protease ATP-binding subunit ClpA (inferred from 100% identity to son:SO_2626)

MetaCyc: 70% identical to ATP-dependent Clp protease ATP-binding subunit ClpA (Escherichia coli K-12 substr. MG1655)
Endopeptidase Clp. [EC: 3.4.21.92]

Predicted SEED Role

"ATP-dependent Clp protease ATP-binding subunit ClpA" in subsystem Proteolysis in bacteria, ATP-dependent

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.92

Use Curated BLAST to search for 3.4.21.92

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8EDW5 at UniProt or InterPro

Protein Sequence (755 amino acids)

>SO2626 ATP-dependent Clp protease, ATP-binding subunit ClpA (NCBI ptt file) (Shewanella oneidensis MR-1)
MLNKDLEVTLNLAFQQAREARHEYMTVEHLLLALIDNPAAQEALIACGANLDKLRDEVAS
FIQQTTPIIADMDDDKETQPTLGFQRVLQRAVFHVQSSGRNEVTGANVLVAIFSEQESQA
VYLLRRSDISRLDVVNFISHGFSKNEDSDAHQDQERSEEQAEASEERSMLSQFATNLNQL
AKQGVIDPLIGRDQEIERAIQTLCRRRKNNPLLVGEAGVGKTAIAEGLAYRIVNHQVPEV
MAQATVYSLDLGSLLAGTKYRGDFEKRFKNLLKELSADKHAILFIDEIHTIIGAGAASGG
VMDASNLLKPLLSSGNLRCMGSTTFQEYQSIFEKDRALARRFQKVDINEPSVAETTKILM
GLKSKYEEYHGVRYTQAAINSAAVLSAKHINDRHLPDKAIDVIDEAGARMAMLPQSKRKK
TIGQAEIETIVAKIARIPEKSVSATDKDLLRNLERNLKMVVFGQDKAIETLSSAIRLSRS
GLGTDKKPVGSFLFAGPTGVGKTEVTNQLANCLGMKLVRFDMSEYMESHTVSRLIGAPPG
YVGYDQGGLLTDAVIKNPHCVVLLDEIEKAHPDVYNLLLQVMDHGTLTDNNGRKADFRHV
TLVMTTNAGVQETIRKSIGFTQQDHTQDALSEINRVFSPEFRNRLDSIIWFNHLDMTIIA
KVVDKFLVELQAQLDAKHVVLEVSDEARTLLAQKGYDKSMGARPMARVVTELIKRPLADE
LLFGELEHGGIAHVDAKDGEIVIHAERNEKTTSKA