Protein Info for SO2445 in Shewanella oneidensis MR-1

Name: thiC
Annotation: thiamin biosynthesis protein ThiC (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13667: ThiC-associated
  • amino acids 40 to 121 (82 residues), 101.1 bits, E=2.4e-33
• TIGR00190: phosphomethylpyrimidine synthase
  • amino acids 164 to 616 (453 residues), 717.2 bits, E=3.1e-220
• PF01964: ThiC_Rad_SAM
  • amino acids 166 to 612 (447 residues), 604 bits, E=1.4e-185

Best Hits

Swiss-Prot: 100% identical to THIC_SHEON: Phosphomethylpyrimidine synthase (thiC) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K03147, thiamine biosynthesis protein ThiC (inferred from 100% identity to son:SO_2445)

MetaCyc: 77% identical to phosphomethylpyrimidine synthase (Escherichia coli K-12 substr. MG1655)
PYRIMSYN1-RXN [EC: 4.1.99.17]

Predicted SEED Role

"Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17)" (EC 4.1.99.17)

MetaCyc Pathways

• superpathway of thiamine diphosphate biosynthesis I (10/10 steps found)
• superpathway of thiamine diphosphate biosynthesis II (9/11 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis I (2/2 steps found)
• superpathway of thiamine diphosphate biosynthesis III (eukaryotes) (3/7 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.99.17

Sequence Analysis Tools

See Q8EED7 at UniProt or InterPro

Protein Sequence (721 amino acids)

>SO2445 thiamin biosynthesis protein ThiC (NCBI ptt file) (Shewanella oneidensis MR-1)
MSSLESSSESIAVNILGQTNRRVARAQAQAFIDTLKPLQHPNSQKLYLQGSRADLRVGMR
QILQTDTRIGGRAAAPNVEKNPPIPVYDCAGPYSDPAAQINVRQGLAKLRLPWIIERQDT
EVLDCTTSDFTQQRLKDDGLDHLRFEAGSSAIIRPRRALPGKRVSQLHYARQGIITPEME
YVAIRENMALADVQDEILNRRGQGESFGAVIGKPITPEFVRDEIARGRAIIPLNINHPEA
EPMTIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWI
IRNSPVPIGTVPIYQALEKVNGVAEHLTWEVFRDTLIEQAEQGVDYFTIHAGVLLRYVPM
TAKRVTGIVSRGGSIMAKWCLSHHQENFLYTHFREICELCVAYDVSLSLGDGMRPGSIAD
ANDEAQFAELETLGELVNIAWEYDVQTIIEGPGHIPMQLIKENMDKQLIHCAEAPFYTLG
PQITDIAPGYDHFTSGIGAAMIAWYGCAMLCYVTPKEHLGLPNKQDVKQGLIAYKIAAHA
ADIAKGHPGAQIRDNALSKARFEFRWEDQYNLGLDPDTARAYHDESLPQESAKVAHFCSM
CGPKFCSMKITQDVRDYAASLEADAKAQSLAAETDGALAPLGSVQQVKSGHSELSHIDAE
LNYIAVKTQDELTAAMAQKSAEFAALGAKLYLPLDNANAANELAKHKDGAKLQSKTQVAK
V