Protein Info for SO2444 in Shewanella oneidensis MR-1

Name: thiDE
Annotation: phosphomethylpyrimidine kinase/thiamin-phosphate pyrophosphorylase, putative (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF08543: Phos_pyr_kin
  • amino acids 24 to 289 (266 residues), 196.2 bits, E=6.3e-62
• PF02581: TMP-TENI
  • amino acids 335 to 505 (171 residues), 173.1 bits, E=3.9e-55
• TIGR00693: thiamine-phosphate diphosphorylase
  • amino acids 336 to 517 (182 residues), 161.4 bits, E=8.1e-52

Best Hits

KEGG orthology group: K14153, hydroxymethylpyrimidine kinase / phosphomethylpyrimidine kinase / thiamine-phosphate diphosphorylase [EC: 2.5.1.3 2.7.1.49 2.7.4.7] (inferred from 100% identity to son:SO_2444)

Predicted SEED Role

No annotation

MetaCyc Pathways

• superpathway of thiamine diphosphate biosynthesis I (10/10 steps found)
• superpathway of thiamine diphosphate biosynthesis II (9/11 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis I (2/2 steps found)
• hydroxymethylpyrimidine salvage (2/2 steps found)
• thiamine diphosphate biosynthesis I (E. coli) (2/2 steps found)
• thiamine diphosphate biosynthesis II (Bacillus) (2/2 steps found)
• thiamine diphosphate salvage II (4/5 steps found)
• thiamine diphosphate salvage V (2/3 steps found)
• thiamine diphosphate biosynthesis III (Staphylococcus) (1/3 steps found)
• thiamine diphosphate biosynthesis IV (eukaryotes) (1/3 steps found)
• superpathway of thiamine diphosphate biosynthesis III (eukaryotes) (3/7 steps found)
• thiamine diphosphate salvage IV (yeast) (3/7 steps found)
• thiamine diphosphate formation from pyrithiamine and oxythiamine (yeast) (3/8 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis II (2/8 steps found)

KEGG Metabolic Maps

• Thiamine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.3 or 2.7.1.49 or 2.7.4.7

Sequence Analysis Tools

See Q8EED8 at UniProt or InterPro

Protein Sequence (526 amino acids)

>SO2444 phosphomethylpyrimidine kinase/thiamin-phosphate pyrophosphorylase, putative (NCBI ptt file) (Shewanella oneidensis MR-1)
MMTPTKHFNHLVPKPIVWTIAGSDSGGGAGIQADLATINDLGGHGCSVITTLTAQSSVAV
DLVEPVSEAMLLTQLSTLLADLPPQAIKIGLLADQQQLQLLADWLANFKTHYPHVPVILD
PVMVASCGDELGNRSTPLDFSRFKGLISLITPNVKELARLTSFTDAQTIPSKMTKADFAA
AAIQLAEQLGCSVLAKGGDNRFDPHLAEDLLVCHDVAGCSELDSHGHFWLVSDRIDTVHN
HGSGCTLSSAIASVLAFGFVLHDAIVVAKAYVNQGLTHARGLGHGPGPLARTTWPHSLLQ
YPKIVAVCADKQPQPYAFKRLTTDLGVYPVVNNLLLLEQLLAAGVKTIQLRVKSDAGITA
AELEAQIQTAIALGKHYDAQLFINDHWQLAIKHGAFGVHLGQEDLAMADLNAIHGAGLAL
GISSHSYFELLRAHQHAPSYIALGHIFPTTTKQMPSAPQGLFKLAHYVELLKAYYPLVAI
GGIGPSNLDQVNATGVRNIAVVRAITEADDPIAALAELTRAWEQGR