Protein Info for SO2395 in Shewanella oneidensis MR-1

Updated annotation (from data): Acyl-CoA dehydrogenase (EC 1.3.8.7)
Rationale: Specific phenotype: utilization of Tween 20. Not sure about preferred substrate length
Original annotation: acyl-CoA dehydrogenase family protein (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 585 PF12418: AcylCoA_DH_N" amino acids 4 to 33 (30 residues), 30.3 bits, see alignment (E = 1.2e-10) PF02771: Acyl-CoA_dh_N" amino acids 81 to 159 (79 residues), 38.5 bits, see alignment E=4.7e-13 PF02770: Acyl-CoA_dh_M" amino acids 163 to 269 (107 residues), 66.4 bits, see alignment E=7.3e-22 PF00441: Acyl-CoA_dh_1" amino acids 279 to 443 (165 residues), 74.7 bits, see alignment E=3.3e-24 PF22924: ACOX_C_alpha1" amino acids 288 to 440 (153 residues), 39.7 bits, see alignment E=1.6e-13 PF08028: Acyl-CoA_dh_2" amino acids 295 to 439 (145 residues), 30.3 bits, see alignment E=1.7e-10 PF12806: Acyl-CoA_dh_C" amino acids 479 to 581 (103 residues), 71.5 bits, see alignment E=2.6e-23

Best Hits

Swiss-Prot: 45% identical to DMDC_RUEPO: 3-methylmercaptopropionyl-CoA dehydrogenase (dmdC) from Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3)

KEGG orthology group: None (inferred from 100% identity to son:SO_2395)

MetaCyc: 45% identical to 3-(methylsulfanyl)propanoyl-CoA dehydrogenase monomer (Ruegeria pomeroyi DSS-3)
RXN-12572 [EC: 1.3.99.41]

Predicted SEED Role

No annotation

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.3.8.7

Use Curated BLAST to search for 1.3.8.7 or 1.3.99.41

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8EEI7 at UniProt or InterPro

Protein Sequence (585 amino acids)

>SO2395 Acyl-CoA dehydrogenase (EC 1.3.8.7) (Shewanella oneidensis MR-1)
MNPYQAPLADMRFLLEQVFDAPNTWSQLPDIAEMVDMDTANAILEEAAKISAQLIHPLNR
AGDEQGVLHQDNCVITPEGYKAVYDQYSQGGWVGLCGEPEFGGMGMPKMLGVLVDEMAYS
ACNAFTLYGSLTAGAALCINAHGSEVLKQAYLPKLYSGEWAGAMDMTEPQAGSDLRNIRT
RAVPQEDGSYAISGSKIFITGGDHDLTENVIHLVLAKLPESKGISLFLVPKITVNKDGCL
GGTNGVSVGSIEHKMGLKGSATCVMNFDEAKGYLIGEPNRGLVCMFTMMNYERLAIGIQG
LGSAQAAYQMATDYAKERSQGVAAGGSPTGSDSDPIIVHGDVRRMLLTIRAMTEAGRALS
VFTGKQLDLAKYAQDDVKAKAARYVGLLTPVAKAFLTDRGLDATIMAQQVFGGHGYIRET
GVEQLVRDTRIAQIYEGTNGIQAIDFLGRKVTGDNLATLKEFVAELKAQMREFTQVDGVK
IAAVCARLDALVNVAESVNEQKLTQPALINACAVDFLDAFGYSVYGFYWLAMLDKVPSAE
DQNFAEQKAYLAKFYFDKLLPKADYHLAQVRAGDVSTMAMPAELF