Protein Info for SO2121 in Shewanella oneidensis MR-1

Name: cheA
Annotation: chemotaxis protein CheA (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 702 PF01627: Hpt" amino acids 8 to 98 (91 residues), 68.4 bits, see alignment E=1.1e-22 PF02895: H-kinase_dim" amino acids 319 to 381 (63 residues), 64 bits, see alignment E=2.8e-21 PF02518: HATPase_c" amino acids 429 to 565 (137 residues), 57.9 bits, see alignment E=2.7e-19 PF01584: CheW" amino acids 571 to 699 (129 residues), 100.7 bits, see alignment E=1.1e-32

Best Hits

KEGG orthology group: K03407, two-component system, chemotaxis family, sensor kinase CheA [EC: 2.7.13.3] (inferred from 100% identity to son:SO_2121)

Predicted SEED Role

"Signal transduction histidine kinase CheA (EC 2.7.3.-)" in subsystem Bacterial Chemotaxis or Flagellar motility or Two-component regulatory systems in Campylobacter (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8EF66 at UniProt or InterPro

Protein Sequence (702 amino acids)

>SO2121 chemotaxis protein CheA (NCBI ptt file) (Shewanella oneidensis MR-1)
MSINMAEFHQVFFEESHEHLENMEQLLIAINLQSPDPEELNTIFRAAHSIKGGSGIFGFT
ALSSVTHVMENLLDKVRKGTFELSSGIIDLLLKTVDTLSHILSLYREEEPIDWQQVEFAK
NQLVAALNGDPFLTTSPEAIPATPTKTEITQTAVVVSDASHQDDIGFGFFEDDVELTLAI
ENQDFGFFDEAFTAKEISVEDINKTLLDSQLVTNTEPDDDLGFGFFESLTPESVDSEINA
LVHIQANKAIAAIEPLSKPVNSPSVPKIPRYTGNTAESTKVAPADPVDPSPVLNKSVASG
TKSTPTAATKKGNTSTQDATLRVETSKIDTLVNLAGELVITQSMLTLIGNEISGELGERL
KTALVELERNTREMQEAVMSVRMLPVSFVFNRFHRLVRDLSDQLGKNVNLVIEGGNTEID
KGMIEKLVDPLTHLVRNSLDHGIEKPEVRRLLGKAEIAQLSLRASQRGGNIVIAVHDDGA
GLHREKILQKARENNMSVTDNMPDKQIWQLIFAAGFSTAKEITDVSGRGVGMDVVRRNIE
ALGGRIDIDSVAGQGATFEIQLPLTLAIVDGMSVSVGKQIYILPLVHIIESIQPHTEQLK
YLAQERLIRVREEYLPLLNLHQLMEITPFAKCPEEGIVVLLESNNKRFGLCVDALVGQQQ
VVIKSLEKHYRRIPGVSGATIMGDGSVALILDVESLAQQIKN