Protein Info for SO1682 in Shewanella oneidensis MR-1

Name: mmsB
Annotation: 3-hydroxyisobutyrate dehydrogenase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 signal peptide" amino acids 1 to 18 (18 residues), see Phobius details PF03807: F420_oxidored" amino acids 3 to 87 (85 residues), 26.6 bits, see alignment E=1.2e-09 PF03446: NAD_binding_2" amino acids 3 to 165 (163 residues), 172.6 bits, see alignment E=9.9e-55 TIGR01692: 3-hydroxyisobutyrate dehydrogenase" amino acids 6 to 293 (288 residues), 399.8 bits, see alignment E=3.6e-124 PF14833: NAD_binding_11" amino acids 168 to 292 (125 residues), 92.2 bits, see alignment E=4.6e-30

Best Hits

Swiss-Prot: 57% identical to SERDH_PSEAE: NAD-dependent L-serine dehydrogenase (PA0743) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00020, 3-hydroxyisobutyrate dehydrogenase [EC: 1.1.1.31] (inferred from 100% identity to son:SO_1682)

MetaCyc: 50% identical to 3-hydroxyisobutyrate dehydrogenase subunit (Pseudomonas putida)
3-hydroxyisobutyrate dehydrogenase. [EC: 1.1.1.31]

Predicted SEED Role

"3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31)" in subsystem Isobutyryl-CoA to Propionyl-CoA Module or Valine degradation (EC 1.1.1.31)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.31

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8EGC2 at UniProt or InterPro

Protein Sequence (300 amino acids)

>SO1682 3-hydroxyisobutyrate dehydrogenase (NCBI ptt file) (Shewanella oneidensis MR-1)
MSTVAFIGLGNMGGPMAANLLKAGMTVRVFDLVHTAMQTLAEQGALVSSTACGAAAGANV
VITMLPAGKHVKNLYLGSGSEKGLLDVVASDTLLIDCSTIDAQSAQLVATQAAKNGLEFM
DAPVSGGTSGAAAGTLTFICGGSDTAFERAQPVLNAMGKNIFHAGGPGAGQIAKICNNML
LSVLMVGTSEALQMGIDHGLDPKVLSNIMKVSSGGNWTLEKYNPCPGVMENVPSSKGYQG
GFMVDLMVKDLGLSFEAALLSNSSTPMGALARSLYVSHARQGNGHRDFSSIFEQFAPLKK