Protein Info for SO1664 in Shewanella oneidensis MR-1

Name: galE
Annotation: UDP-glucose 4-epimerase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04321: RmlD_sub_bind
  • amino acids 1 to 165 (165 residues), 45.6 bits, E=2.5e-15
• PF08659: KR
  • amino acids 2 to 131 (130 residues), 36.7 bits, E=2.1e-12
• PF00106: adh_short
  • amino acids 2 to 113 (112 residues), 33.7 bits, E=1.3e-11
• TIGR01179: UDP-glucose 4-epimerase GalE
  • amino acids 3 to 334 (332 residues), 475.5 bits, E=3.6e-147
• PF01370: Epimerase
  • amino acids 3 to 261 (259 residues), 198.5 bits, E=6.1e-62
• PF02719: Polysacc_synt_2
  • amino acids 3 to 183 (181 residues), 60.2 bits, E=1e-19
• PF01073: 3Beta_HSD
  • amino acids 4 to 157 (154 residues), 60.3 bits, E=8e-20
• PF16363: GDP_Man_Dehyd
  • amino acids 4 to 321 (318 residues), 202.6 bits, E=6.1e-63
• PF07993: NAD_binding_4
  • amino acids 5 to 174 (170 residues), 35.9 bits, E=2.4e-12
• PF13460: NAD_binding_10
  • amino acids 7 to 177 (171 residues), 29.4 bits, E=3.6e-10

Best Hits

Swiss-Prot: 69% identical to GALE_BACSU: UDP-glucose 4-epimerase (galE) from Bacillus subtilis (strain 168)

KEGG orthology group: K01784, UDP-glucose 4-epimerase [EC: 5.1.3.2] (inferred from 100% identity to son:SO_1664)

MetaCyc: 69% identical to UDP-glucose 4-epimerase (Bacillus subtilis subtilis 168)
UDP-N-acetylglucosamine 4-epimerase. [EC: 5.1.3.7]; UDP-glucose 4-epimerase. [EC: 5.1.3.7, 5.1.3.2]

Predicted SEED Role

"UDP-glucose 4-epimerase (EC 5.1.3.2)" in subsystem Lacto-N-Biose I and Galacto-N-Biose Metabolic Pathway or Lactose and Galactose Uptake and Utilization or N-linked Glycosylation in Bacteria or Rhamnose containing glycans or linker unit-arabinogalactan synthesis (EC 5.1.3.2)

MetaCyc Pathways

• D-galactose degradation I (Leloir pathway) (4/5 steps found)
• UDP-α-D-galactose biosynthesis (1/1 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis I (1/1 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (5/7 steps found)
• D-galactose detoxification (2/3 steps found)
• superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis (4/6 steps found)
• UDP-α-D-galactofuranose biosynthesis (1/2 steps found)
• colanic acid building blocks biosynthesis (6/11 steps found)
• stachyose degradation (3/7 steps found)
• UDP-sugars interconversion (2/9 steps found)
• teichuronic acid biosynthesis (B. subtilis 168) (2/9 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)
• mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (1/18 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Galactose metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 5.1.3.2, 5.1.3.7

Use Curated BLAST to search for 5.1.3.2 or 5.1.3.7

Sequence Analysis Tools

See Q8EGE0 at UniProt or InterPro

Protein Sequence (337 amino acids)

>SO1664 UDP-glucose 4-epimerase (NCBI ptt file) (Shewanella oneidensis MR-1)
MTILVTGGAGYIGTHTVVELLNAGSEVIVLDNLSNSSIEALDRVERITGKSVTFYQGDIL
NKALLQKVFSDHSIDAVIHFAGLKAVGESVAKPLKYYENNVTGTLILCQVMAEFKVKNLV
FSSSATVYGDPASLPITEDFPTGATNPYGQSKLMVEHILADLHHSDPSWNIARLRYFNPV
GAHASGLIGEDPNDIPNNLMPFIAQVAVGKREALSVFGNDYPTHDGTGVRDYIHVVDLAI
GHLKALEKLATKPGLVTYNLGTGQGYSVLDMVKAFEKACGKSIAYLIAPRRPGDIAACYA
DPDHAKTDLDWQATHSLEDMANSSWHWQSTNPNGYKG