Protein Info for SO1494 in Shewanella oneidensis MR-1

Name: glgB
Annotation: 1,4-alpha-glucan branching enzyme (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 746 TIGR01515: 1,4-alpha-glucan branching enzyme" amino acids 115 to 733 (619 residues), 890.2 bits, see alignment E=3.7e-272 PF02922: CBM_48" amino acids 131 to 213 (83 residues), 79.2 bits, see alignment E=3.6e-26 PF00128: Alpha-amylase" amino acids 278 to 352 (75 residues), 29.5 bits, see alignment E=8.6e-11 PF02806: Alpha-amylase_C" amino acids 637 to 733 (97 residues), 80.5 bits, see alignment E=1.5e-26

Best Hits

Swiss-Prot: 100% identical to GLGB_SHEON: 1,4-alpha-glucan branching enzyme GlgB (glgB) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K00700, 1,4-alpha-glucan branching enzyme [EC: 2.4.1.18] (inferred from 100% identity to son:SO_1494)

Predicted SEED Role

"1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 2.4.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8EGU7 at UniProt or InterPro

Protein Sequence (746 amino acids)

>SO1494 1,4-alpha-glucan branching enzyme (NCBI ptt file) (Shewanella oneidensis MR-1)
MMTQAQTYFYDGSDVALLNGQYTDVFSLLGMHSINEGKALVVRCFLRNAQKVDVISLKDG
RKVASLERVNEAGLFAGTLGRRIKPFLYALRVTYPLCELDIIDPYQFGSLLNREDLYLFG
EGSSEQAYRFLGANWRQVDNVEGVHFCVWAPNAKRVSVVGDFNHWDDTRHVMRQHVANGL
WEIFLPGVVEGSHYKFDLVYQNGERHAKSDPMATQMECAPHNASIVPKKQQHQWADTQWM
DKRAATAWHRAPMSIYEVQLGSWRRKGEFGEQYFDYQDLIEQLIPYVKEQGFTHIELMPV
SEYPFDGSWGYQPVGLYAPTYRFGDANGFKAFIDACHQAEIGVLLDWVAAHFPKDPHGLV
RFDGTCLYEHEDPRKGTHPDWDTLIYNYGRGEVRSFLLSNACYWLREFHLDGLRLDAVSS
MLYLDYSREPGQWLPNAYGGRENLEAIDFLQMLNQRLYQAIPGICMIAEESTAFAGVTKP
TDSSGLGFGFKWNMGWMNDSLSYLGRDPIYRQYHHNQLTFSLMYAYSEQFMLSISHDEVV
HGKGSLLHKIPGDDWQKFATLRAYYGFMWGHPGKKLLFMGSEFAQRDEWDHDHSLDWHLL
AFEPHQGVQRWLRDLNQLYRQFPALSVLDYEPQGFRWLDCDNGRDSIFSFVRYGEGNDVP
LVFVVNMTPTVHQGFRIGLPQGGDFCEYLNSDSYLYGGSNQGNGGKIIAQALPWQGMASS
ALITVPPLGCLILGPATENILRESAL