Protein Info for SO1483 in Shewanella oneidensis MR-1

Name: aceB
Updated annotation (from data): Malate synthase (EC 2.3.3.9)
Rationale: Specifically important for utilizing Tween 20. Automated validation from mutant phenotype: the predicted function (MALSYN-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: malate synthase A (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF20656: MS_N
  • amino acids 33 to 83 (51 residues), 45.6 bits, 6.2e-16
• TIGR01344: malate synthase A
  • amino acids 37 to 547 (511 residues), 815.9 bits, E=5.1e-250
• PF01274: MS_TIM-barrel
  • amino acids 176 to 422 (247 residues), 336.1 bits, E=1.4e-104
• PF20659: MS_C
  • amino acids 427 to 546 (120 residues), 138.9 bits, E=1.7e-44

Best Hits

Swiss-Prot: 70% identical to MASY_ECOLI: Malate synthase A (aceB) from Escherichia coli (strain K12)

KEGG orthology group: K01638, malate synthase [EC: 2.3.3.9] (inferred from 100% identity to son:SO_1483)

MetaCyc: 70% identical to malate synthase A (Escherichia coli K-12 substr. MG1655)
Malate synthase. [EC: 2.3.3.9]

Predicted SEED Role

"Malate synthase (EC 2.3.3.9)" in subsystem Photorespiration (oxidative C2 cycle) (EC 2.3.3.9)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
• glyoxylate cycle (6/6 steps found)
• superpathway of glyoxylate bypass and TCA (10/12 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• glycolate and glyoxylate degradation II (2/2 steps found)
• chitin deacetylation (3/4 steps found)
• superpathway of glycol metabolism and degradation (5/7 steps found)
• L-arabinose degradation IV (2/8 steps found)
• D-xylose degradation IV (1/7 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (1/14 steps found)
• superpathway of pentose and pentitol degradation (4/42 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.3.9

Use Curated BLAST to search for 2.3.3.9

Sequence Analysis Tools

See Q8EGV8 at UniProt or InterPro

Protein Sequence (549 amino acids)

>SO1483 Malate synthase (EC 2.3.3.9) (Shewanella oneidensis MR-1)
MTEHTLSEQQVNLTLNKATANGTLALVGNTIPGQEVIFTEGAMALLESLCREFGAEVPTL
LAKRKDRQARIDKGALPDFLPETRAIRDGAWKIRGIPNDLLDRRVEITGPVERKMVINAL
NANAKVFMADFEDSLAPSWQKVVEGQINLRDAVRGEIEYTAPETGKHYKLGPNPAVLICR
VRGLHLKEKHVEFNQQSIPGGLFDFAMYFYHNYRQLLAKGSGPYFYIPKLESHIEARWWA
KVFAFVEERFCLQAGTIKCTCLIETLPAVFEMDEILYELRSNIVALNCGRWDYIFSYIKT
LKRHGDRVLPDRQAVTMDTPFLSAYSRLLIKTCHKRGALAMGGMAAFIPAKDPAQNEAVL
QRVRKDKELEARNGHDGTWVAHPGLADTAMGIFNEYIGQDHQNQLHITRDVDAPILAAEL
LKTCDGERTEQGMRLNIRIALQYLEAWISGNGCVPIYGLMEDAATAEISRASIWQWIQHG
KSLSNGKLVTKQLFKDMLVEELANVKKEVGSDRFTHGKFTQAAVLLEDITTSDELVDFLT
LPGYEMLTA