Protein Info for SO1322 in Shewanella oneidensis MR-1

Name: pfs
Annotation: 5-methylthioadenosine nucleosidase/S-adenosylhomocysteine nucleosidase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01704: MTA/SAH nucleosidase
  • amino acids 2 to 229 (228 residues), 271.9 bits, E=2e-85
• PF01048: PNP_UDP_1
  • amino acids 2 to 206 (205 residues), 180 bits, E=2.5e-57

Best Hits

Swiss-Prot: 100% identical to MTNN_SHEON: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K01243, S-adenosylhomocysteine/5'-methylthioadenosine nucleosidase [EC: 3.2.2.9] (inferred from 100% identity to son:SO_1322)

MetaCyc: 56% identical to 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase monomer (Vibrio cholerae)
Methylthioadenosine nucleosidase. [EC: 3.2.2.16, 3.2.2.9]; 3.2.2.9 [EC: 3.2.2.16, 3.2.2.9]

Predicted SEED Role

"5'-methylthioadenosine nucleosidase (EC 3.2.2.16) @ S-adenosylhomocysteine nucleosidase (EC 3.2.2.9)" (EC 3.2.2.16, EC 3.2.2.9)

MetaCyc Pathways

• S-adenosyl-L-methionine salvage I (4/4 steps found)
• autoinducer AI-2 biosynthesis II (Vibrio) (5/6 steps found)
• autoinducer AI-2 biosynthesis I (4/5 steps found)
• S-methyl-5'-thioadenosine degradation IV (1/1 steps found)
• S-methyl-5'-thioadenosine degradation I (1/2 steps found)
• L-cysteine biosynthesis VI (reverse transsulfuration) (4/7 steps found)
• 5'-deoxyadenosine degradation II (1/4 steps found)
• L-methionine salvage cycle I (bacteria and plants) (6/12 steps found)
• L-methionine salvage cycle II (plants) (4/11 steps found)

KEGG Metabolic Maps

• Methionine metabolism
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 3.2.2.16, 3.2.2.9

Use Curated BLAST to search for 3.2.2.16 or 3.2.2.9

Sequence Analysis Tools

See Q8EHA7 at UniProt or InterPro

Protein Sequence (236 amino acids)

>SO1322 5-methylthioadenosine nucleosidase/S-adenosylhomocysteine nucleosidase (NCBI ptt file) (Shewanella oneidensis MR-1)
MKIGIIGAMEPEVAHLIAAMTNTSSQTIADIEFIAGTLAGKDVVVTRSGIGKVAASIATT
LLIEKYAPDAVINTGSAGGFVDSLSIGDIVISSEVRHHDVDVTAFGYEIGQMAQQPAAFI
PAPYLVEAANKAIAQLGEVRAIEGLICTGDSFICDPVRTKTMLEHFPTMAACEMEGAAIA
QVCHQFGVPFVVIRSLSDNANNDSPVDFDAYIVKAGYHSALMVMLLLEQLEPNSVK