Protein Info for SO1209 in Shewanella oneidensis MR-1

Name: pnp
Annotation: polyribonucleotide nucleotidyltransferase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 702 TIGR03591: polyribonucleotide nucleotidyltransferase" amino acids 12 to 693 (682 residues), 1077.2 bits, see alignment E=0 PF01138: RNase_PH" amino acids 17 to 146 (130 residues), 111.9 bits, see alignment E=9.2e-36 amino acids 326 to 458 (133 residues), 90.5 bits, see alignment E=3.8e-29 PF03725: RNase_PH_C" amino acids 150 to 204 (55 residues), 44.1 bits, see alignment 4.7e-15 amino acids 463 to 529 (67 residues), 26 bits, see alignment E=2.2e-09 PF03726: PNPase" amino acids 244 to 322 (79 residues), 71.8 bits, see alignment E=1.6e-23 PF00013: KH_1" amino acids 558 to 616 (59 residues), 40.4 bits, see alignment 6.2e-14 PF00575: S1" amino acids 621 to 692 (72 residues), 67.8 bits, see alignment E=2.6e-22

Best Hits

Swiss-Prot: 100% identical to PNP_SHEON: Polyribonucleotide nucleotidyltransferase (pnp) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K00962, polyribonucleotide nucleotidyltransferase [EC: 2.7.7.8] (inferred from 100% identity to son:SO_1209)

MetaCyc: 73% identical to polynucleotide phosphorylase (Escherichia coli K-12 substr. MG1655)
Exoribonuclease II. [EC: 3.1.13.1]; Polyribonucleotide nucleotidyltransferase. [EC: 3.1.13.1, 2.7.7.8]

Predicted SEED Role

"Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8)" in subsystem Bacterial RNA-metabolizing Zn-dependent hydrolases or Polyadenylation bacterial (EC 2.7.7.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.13.1

Use Curated BLAST to search for 2.7.7.8 or 3.1.13.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8EHL1 at UniProt or InterPro

Protein Sequence (702 amino acids)

>SO1209 polyribonucleotide nucleotidyltransferase (NCBI ptt file) (Shewanella oneidensis MR-1)
MGHVNPIVKSFEYGQHTVTLETGVIARQADAAVLASMGDTTVLVTVVGKKEAEAGRDFFP
LTVNYQEKTYAAGKIPGGFFKREGRPSEEETLIARLIDRPIRPLFPNGFKNEVQVIITVV
SVDPEIEPDIISMIGTSAALAISGIPFNGPLGAARVGYINGEYVLNPTVKQIGASQLNLV
VAGTESAVLMVESEAQALPEEVMLGSVVYGHDQQQVVIKAIAEFKAEAGKPTWDWTAPVE
DEALVAQIKELAEAGFIEAYQIQVKQERYAQVAVVKAAAKEALLAANPDVDLREVDNLLG
SLEKKVVRGRILRGMPRIDGREPDMVRALSVLAGVLPRTHGSALFTRGETQALVTCTLGT
ERDAQKIDSIMGERTNRFMLHYNFPPYSVGETGMVGSPKRREIGHGKLAWRGINAVMPSA
EEFPYSVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDDFVVL
SDILGDEDHLGDMDFKVAGTRDGVTALQMDIKIEGITKEIMEIALQQAYGARVHILNVMD
QAIGSHRADISDHAPRITTIKINPEKIRDVIGKGGAVIRALTEETGTTIELEDDGTVKIA
SSNGEATKEAIRRIEEITSEVEVGRIYNGKVIRIVDFGAFVNILPGKDGLVHISQISDER
VANVSDHLELNQEVTVKVMEVDRQGRVRLSIKEAQTKEPAAE