Protein Info for SO1141 in Shewanella oneidensis MR-1

Name: carA
Annotation: carbamoyl-phosphate synthase, small subunit (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01368: carbamoyl-phosphate synthase, small subunit
  • amino acids 9 to 379 (371 residues), 489.6 bits, E=2.6e-151
• PF00988: CPSase_sm_chain
  • amino acids 11 to 136 (126 residues), 177.2 bits, E=1.8e-56
• PF00117: GATase
  • amino acids 200 to 376 (177 residues), 192.5 bits, E=9.1e-61
• PF07722: Peptidase_C26
  • amino acids 254 to 287 (34 residues), 22.7 bits, 1.1e-08

Best Hits

Swiss-Prot: 100% identical to CARA_SHEON: Carbamoyl-phosphate synthase small chain (carA) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K01956, carbamoyl-phosphate synthase small subunit [EC: 6.3.5.5] (inferred from 100% identity to son:SO_1141)

MetaCyc: 76% identical to carbamoyl-phosphate synthetase small subunit (Escherichia coli K-12 substr. MG1655)
Carbamoyl-phosphate synthase (glutamine-hydrolyzing). [EC: 6.3.5.5]

Predicted SEED Role

"Carbamoyl-phosphate synthase small chain (EC 6.3.5.5)" in subsystem De Novo Pyrimidine Synthesis or Macromolecular synthesis operon (EC 6.3.5.5)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
• L-arginine biosynthesis II (acetyl cycle) (9/10 steps found)
• UMP biosynthesis I (6/6 steps found)
• UMP biosynthesis II (6/6 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
• L-citrulline biosynthesis (7/8 steps found)
• L-glutamate and L-glutamine biosynthesis (6/7 steps found)
• L-citrulline degradation (3/3 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• superpathway of arginine and polyamine biosynthesis (13/17 steps found)
• UMP biosynthesis III (5/6 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-arginine degradation V (arginine deiminase pathway) (3/4 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (1/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (1/4 steps found)
• L-arginine biosynthesis IV (archaea) (4/9 steps found)
• allantoin degradation IV (anaerobic) (2/9 steps found)
• guadinomine B biosynthesis (2/13 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.5

Use Curated BLAST to search for 6.3.5.5

Sequence Analysis Tools

See Q8EHS6 at UniProt or InterPro

Protein Sequence (386 amino acids)

>SO1141 carbamoyl-phosphate synthase, small subunit (NCBI ptt file) (Shewanella oneidensis MR-1)
MEVALTKSALLVLEDGTVFTGTAIGADGLSVGEVVFNTSMTGYQEILTDPSYSRQIVTLT
YPHIGNTGTNNEDVESNGVHACGLIIRDLPLIASSFRNQQNLSDYLKANNVVGIADIDTR
KLTRILREKGAQAGCIMAGADLDEVKALAAAKAFPGLKGMDLAKEVTTDKAYPWRKGSWR
LVGGLPADTPAEALKYKVVAYDYGVKQNILRMLVDRGCDVTVVPAKTPASEVLAMNPDGV
FLSNGPGDPEPCDYAIEAIQQILKTDTPIFGICLGHQLLALASGAKTLKMKFGHHGANHP
VSNIEKGNVMITSQNHGFAADEATLPANIKVTHKSLFDGSLQGIHLTDKPAFSFQGHPEA
SPGPNDAAPLFDHFIELIEHYRQNAK