Protein Info for SO1019 in Shewanella oneidensis MR-1

Name: nuoCD
Annotation: NADH dehydrogenase I, C/D subunits (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01961: NADH (or F420H2) dehydrogenase, subunit C
  • amino acids 50 to 177 (128 residues), 127.5 bits, E=4.2e-41
• PF00329: Complex1_30kDa
  • amino acids 51 to 179 (129 residues), 139.5 bits, E=9e-45
• TIGR01962: NADH dehydrogenase (quinone), D subunit
  • amino acids 216 to 601 (386 residues), 567.1 bits, E=1.8e-174
• PF00346: Complex1_49kDa
  • amino acids 330 to 601 (272 residues), 360.5 bits, E=4.7e-112

Best Hits

Swiss-Prot: 100% identical to NUOCD_SHEON: NADH-quinone oxidoreductase subunit C/D (nuoC) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K13378, NADH dehydrogenase I subunit C/D [EC: 1.6.5.3] (inferred from 100% identity to son:SO_1019)

MetaCyc: 77% identical to NADH:quinone oxidoreductase subunit CD (Escherichia coli K-12 substr. MG1655)
NADH-DEHYDROG-A-RXN [EC: 7.1.1.2]; 7.1.1.- [EC: 7.1.1.2]

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain C (EC 1.6.5.3) / NADH-ubiquinone oxidoreductase chain D (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

• NADH to cytochrome bd oxidase electron transfer I (2/2 steps found)
• NADH to dimethyl sulfoxide electron transfer (2/2 steps found)
• NADH to fumarate electron transfer (2/2 steps found)
• aerobic respiration I (cytochrome c) (3/4 steps found)
• aerobic respiration III (alternative oxidase pathway) (2/3 steps found)
• NADH to cytochrome bo oxidase electron transfer I (1/2 steps found)
• NADH to hydrogen peroxide electron transfer (1/2 steps found)
• NADH to trimethylamine N-oxide electron transfer (1/2 steps found)
• nitrate reduction VIII (dissimilatory) (1/2 steps found)
• Fe(II) oxidation (3/6 steps found)
• NAD(P)/NADPH interconversion (3/6 steps found)

KEGG Metabolic Maps

• Oxidative phosphorylation

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3 or 7.1.1.2

Sequence Analysis Tools

See Q8EI31 at UniProt or InterPro

Protein Sequence (601 amino acids)

>SO1019 NADH dehydrogenase I, C/D subunits (NCBI ptt file) (Shewanella oneidensis MR-1)
MKLTRDFPHNDTMAQWQPSDHKDAKVVGELFAHFGAEHFTVQTTRTGVPVVWLPRELLQD
VVGFLRKLPAPFVMLYDLSATDERLRSHRDGLPQSDFTVFYHLISIDRNADVMLKVALAE
SDLHLPTITNHFPNANWYEREVWDLMGITFDGHPHLTRIMMPKSWQGHPLRKDYPARATE
FDPFMLDAAKQDMEQENLLFKPEEWGLARGNENEDYMFLNLGPNHPSAHGAFRLVLQLDG
EEVRNCVPDIGYHHRGAEKMGERQSWHSYIPYTDRVEYLGGVMNNLPYVLAVEKLAGIKV
PQRVDMIRVMMAELFRIQSHLLFLGTYIQDVGAMTPVFFTFTDRQHIYTIIEAITGARMH
PAWFRIGGVAHDLPKGWQRLVQDNLLSWLPKRLMDYEKAAMRNSILRGRTIGVAAYTTEQ
ALAWGTTGAGLRATGLNFDVRKWRPYSGYEQFDFEVPVGSNGDAYDRATVRIEEIRQSMR
IIEQCMNNMPEGPFKADHPLTTPPPKERTLQHIETLINHFLQVSWGPVMPAAESFQMIEA
TKGINSYYLTSDGSTMSYRTRIRTPSFAHLQQIPSVIKGSMVSDLIVYLGSIDFVMSDVD
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