Protein Info for SO1011 in Shewanella oneidensis MR-1

Name: nuoL
Annotation: NADH dehydrogenase I, L subunit (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 615 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details transmembrane" amino acids 29 to 53 (25 residues), see Phobius details amino acids 62 to 82 (21 residues), see Phobius details amino acids 88 to 108 (21 residues), see Phobius details amino acids 117 to 134 (18 residues), see Phobius details amino acids 140 to 160 (21 residues), see Phobius details amino acids 172 to 191 (20 residues), see Phobius details amino acids 210 to 230 (21 residues), see Phobius details amino acids 250 to 269 (20 residues), see Phobius details amino acids 281 to 302 (22 residues), see Phobius details amino acids 309 to 329 (21 residues), see Phobius details amino acids 335 to 357 (23 residues), see Phobius details amino acids 376 to 395 (20 residues), see Phobius details amino acids 415 to 437 (23 residues), see Phobius details amino acids 457 to 478 (22 residues), see Phobius details amino acids 498 to 517 (20 residues), see Phobius details amino acids 529 to 548 (20 residues), see Phobius details amino acids 595 to 614 (20 residues), see Phobius details TIGR01974: proton-translocating NADH-quinone oxidoreductase, chain L" amino acids 3 to 612 (610 residues), 740.7 bits, see alignment E=9.5e-227 PF00662: Proton_antipo_N" amino acids 69 to 119 (51 residues), 69.8 bits, see alignment 1.5e-23 PF00361: Proton_antipo_M" amino acids 135 to 427 (293 residues), 297 bits, see alignment E=1.4e-92

Best Hits

Swiss-Prot: 73% identical to NUOL_PSEAE: NADH-quinone oxidoreductase subunit L (nuoL) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00341, NADH dehydrogenase I subunit L [EC: 1.6.5.3] (inferred from 100% identity to son:SO_1011)

MetaCyc: 74% identical to NADH-quinone oxidoreductase subunit L (Pseudomonas putida KT2440)
NADH-DEHYDROG-A-RXN [EC: 7.1.1.2]

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain L (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3 or 7.1.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8EI39 at UniProt or InterPro

Protein Sequence (615 amino acids)

>SO1011 NADH dehydrogenase I, L subunit (NCBI ptt file) (Shewanella oneidensis MR-1)
MSLLYLTFLFPLLGWLVLAFSLGRFGERTSALIGVGSIGLSALTTLWVGMDFLANPPEGG
VYIQHLWQWMVVGNFTPSFSLALDGLSLTMLGVVTGVGFFIHLFASWYMRGEEGYSRFFT
YTNLFIASMLFLVLADDLLFVYLGWEGVGLCSYLLIGFYYKDRNNGAAALKAFIVTRVGD
VFLAIGLFILYQELGTLNIHELLVRAPTLFAEGSPALSLACLMLLGGAVGKSAQLPLQTW
LADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLLAPEVLHLVGLVGAITLVLAGFAA
LVQTDIKRILAYSTMSQIGYMFLALGVGAWEGAIFHLMTHAFFKALLFLSAGAVIVACHH
EQNIFKMGGLRKSLPLVYACFLVGGSALAALPLVTSGFYSKDAILWQVEASGQSALLWAG
LAGAFLTSLYTFRLIFIAFHGKEQTKAHAGHGLAHHLPLLVLLVLSTGIGALITPPLAGV
LPAGPGDHIEEGRHALEITSGIIAIAGIALAAFLFLGERRLATSIANSAPGRLLSTLWFN
AWGFDWLYDQLWVKPYLLATRLVGQDPLDWMMGLPAWLALRGNQLLAWTVTGNLRWYAAS
IGIGAVLVLALLLLG