Protein Info for SO1009 in Shewanella oneidensis MR-1

Name: nuoN
Annotation: NADH dehydrogenase I, N subunit (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01770: proton-translocating NADH-quinone oxidoreductase, chain N
  • amino acids 9 to 475 (467 residues), 427.9 bits, E=2.9e-132
• PF00361: Proton_antipo_M
  • amino acids 122 to 420 (299 residues), 259.3 bits, E=2.3e-81

Best Hits

Swiss-Prot: 91% identical to NUON_AERHH: NADH-quinone oxidoreductase subunit N (nuoN) from Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240)

KEGG orthology group: K00343, NADH dehydrogenase I subunit N [EC: 1.6.5.3] (inferred from 100% identity to son:SO_1009)

MetaCyc: 61% identical to NADH:quinone oxidoreductase subunit N (Escherichia coli K-12 substr. MG1655)
NADH-DEHYDROG-A-RXN [EC: 7.1.1.2]; 7.1.1.- [EC: 7.1.1.2]

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain N (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

• NADH to cytochrome bd oxidase electron transfer I (2/2 steps found)
• NADH to dimethyl sulfoxide electron transfer (2/2 steps found)
• NADH to fumarate electron transfer (2/2 steps found)
• aerobic respiration I (cytochrome c) (3/4 steps found)
• aerobic respiration III (alternative oxidase pathway) (2/3 steps found)
• NADH to cytochrome bo oxidase electron transfer I (1/2 steps found)
• NADH to hydrogen peroxide electron transfer (1/2 steps found)
• NADH to trimethylamine N-oxide electron transfer (1/2 steps found)
• nitrate reduction VIII (dissimilatory) (1/2 steps found)
• Fe(II) oxidation (3/6 steps found)
• NAD(P)/NADPH interconversion (3/6 steps found)

KEGG Metabolic Maps

• Oxidative phosphorylation

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3 or 7.1.1.2

Sequence Analysis Tools

See Q8EI41 at UniProt or InterPro

Protein Sequence (487 amino acids)

>SO1009 NADH dehydrogenase I, N subunit (NCBI ptt file) (Shewanella oneidensis MR-1)
MTFSASQLLALLPLLLTTGAMAALMLAIAWRRCVTTAFTVTIVGLNMALFSLPIVMAQGD
QGVTPLLQVDSYAVFYMGLVLIGALATCTFGQSWLKAYPDNREEFFLLLLIATAGGLVLA
CSRHLASLFIGIEMLTLPMFGLVGYAYRERHSLEAAVKYMVLSAAATAFLLFGMALLYAQ
AGSLSFAALGQTLAESPAHHPLLMGGLGLMLVGFAFKLSLAPFHLWTPDVYEGAPAPVAT
FLATVSKVAVFAVLLRFYLAVPAASDPMIHWLLAAMAVISILVGNLLALVQTNVKRMLGY
SSISHFGYLLAVIVASRIGQMPVEAAGVYLLMYLFTSLGAFGVVSMMSSPYRGKDADSLH
SYRGLFWKRPYLTAVLTVMMLSLAGIPMTLGFIGKFYLIGVTVEAQLWWLSGAIVLGSAL
GLYYYLKVMVTLYLREPGMQQRDASADWALSSGGVVVLLSAALVVLLGLYPQPVITLVQG
FQHVVLQ