Protein Info for SO0980 in Shewanella oneidensis MR-1

Annotation: RNA methyltransferase, TrmA family (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 382 TIGR02085: 23S rRNA (uracil-5-)-methyltransferase RumB" amino acids 2 to 379 (378 residues), 582.1 bits, see alignment E=4.7e-179 TIGR00479: 23S rRNA (uracil-5-)-methyltransferase RumA" amino acids 3 to 372 (370 residues), 183.9 bits, see alignment E=5e-58 PF05958: tRNA_U5-meth_tr" amino acids 211 to 378 (168 residues), 68.4 bits, see alignment E=1.2e-22 PF05175: MTS" amino acids 239 to 318 (80 residues), 29 bits, see alignment E=1.6e-10 PF09445: Methyltransf_15" amino acids 240 to 318 (79 residues), 24.9 bits, see alignment E=2.7e-09 PF13847: Methyltransf_31" amino acids 240 to 312 (73 residues), 34.9 bits, see alignment E=2.3e-12

Best Hits

Swiss-Prot: 100% identical to RLMC_SHEON: 23S rRNA (uracil(747)-C(5))-methyltransferase RlmC (rlmC) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K03212, RNA methyltransferase, TrmA family [EC: 2.1.1.-] (inferred from 100% identity to son:SO_0980)

MetaCyc: 56% identical to 23S rRNA m5U747 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN-11600 [EC: 2.1.1.189]

Predicted SEED Role

No annotation

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.- or 2.1.1.189

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8EI67 at UniProt or InterPro

Protein Sequence (382 amino acids)

>SO0980 RNA methyltransferase, TrmA family (NCBI ptt file) (Shewanella oneidensis MR-1)
MQCGYFERGQCQSCRHIKLPMVQQLAAKTQELQQLLAPFVVSTNTQFLPPVVGEDSGFRN
KAKMVVLGAAHAPVLGIVSPNGEAVSLCDCLLYPADMQALLYRLERFVQQAGIPPYRIDK
AKGELKYILLTRSQVRGEYMLRFVLRSPDAIARIERELPALMAEYPQINVVSVNIQPIHM
AILEGDEEIFLTENTRLEERFNDVPLFIRPKSFFQTNPHVAAKLYQTAREWVGEFAPKSL
WDLFCGVGGFGLHCASKDIPLTGIEIEAEAIACAKMSAQMMGLDNVQFMALDSTDFAKGD
AAATKPDLIIVNPPRRGIGESLCDALSEFAPRAILYSSCNPKTLAKDLVHIRGYQLTRVQ
LFDLFPHSDHFEVLAMLVKDSR