Protein Info for SO0956 in Shewanella oneidensis MR-1

Name: ahpF
Annotation: alkyl hydroperoxide reductase, F subunit (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 527 TIGR03140: alkyl hydroperoxide reductase subunit F" amino acids 1 to 512 (512 residues), 803.5 bits, see alignment E=3.6e-246 PF13192: Thioredoxin_3" amino acids 123 to 193 (71 residues), 42.3 bits, see alignment E=2.5e-14 PF07992: Pyr_redox_2" amino acids 211 to 498 (288 residues), 168.5 bits, see alignment E=9.4e-53 PF12831: FAD_oxidored" amino acids 212 to 248 (37 residues), 30.1 bits, see alignment 1.3e-10 PF01134: GIDA" amino acids 213 to 251 (39 residues), 19.4 bits, see alignment 2e-07 PF13738: Pyr_redox_3" amino acids 260 to 481 (222 residues), 58.3 bits, see alignment E=3.2e-19 PF00070: Pyr_redox" amino acids 351 to 423 (73 residues), 46.7 bits, see alignment E=1.5e-15

Best Hits

Swiss-Prot: 65% identical to AHPF_PSEPU: Alkyl hydroperoxide reductase subunit F (ahpF) from Pseudomonas putida

KEGG orthology group: K03387, alkyl hydroperoxide reductase subunit F [EC: 1.6.4.-] (inferred from 100% identity to son:SO_0956)

MetaCyc: 59% identical to alkyl hydroperoxide reductase, AhpF component (Escherichia coli K-12 substr. MG1655)
RXN-8506 [EC: 1.5.1.37]; R4-RXN [EC: 1.5.1.37, 1.11.1.26]

Predicted SEED Role

"Alkyl hydroperoxide reductase protein F (EC 1.6.4.-)" in subsystem Thioredoxin-disulfide reductase (EC 1.6.4.-)

Isozymes

Compare fitness of predicted isozymes for: 1.6.4.-

Use Curated BLAST to search for 1.11.1.26 or 1.5.1.37 or 1.6.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8EI88 at UniProt or InterPro

Protein Sequence (527 amino acids)

>SO0956 alkyl hydroperoxide reductase, F subunit (NCBI ptt file) (Shewanella oneidensis MR-1)
MLDANLKNQLQTYLQNLKRPVELVVSADESKKSQELKSLVNDIVSLSTFVSLKEAQGQRT
PAMTVVNPALNTQISFAGLPMGHEFTSLVLALLHSGGHPIKLSDDIIEQIRNLPGKYEFE
TYVSLTCQNCPDVVQALNMMAAINPNITNVMIDGALFQDEVASRNIMAVPSVYLNGEVFA
AGRISIGEILNKLDTGAASRKAEELSQKAPYEVLVVGGGPAGAAAAIYAARKGLRTGIVA
DKFGGQVAETVGIENFISVKATEGPKLVANLEAHVRDYEVDIMDNQKAVKLASDGLFELE
LASGAKLRSRTVLLATGARWREMNVPGEKEYRGKGVAYCPHCDGPLFKGKRVAVIGGGNS
GIEAAIDLANIVEHVTVLEFDSKLRADDVLQRKAASMGNIKIITQAMTTEVTGDGTRVNG
LNYTDRATGESHHIALAGIFVQIGLVPNAEWLKGTVDLTPRGEIIVDERGQTSVPGVFAA
GDVTNSPYKQIIIAMGSGANASLGAFDYLIRHSDDSTETKTTDTKAA