Protein Info for SO0948 in Shewanella oneidensis MR-1

Annotation: conserved hypothetical protein (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 220 PF03602: Cons_hypoth95" amino acids 13 to 102 (90 residues), 34.3 bits, see alignment E=6.7e-12 PF06325: PrmA" amino acids 18 to 96 (79 residues), 26.9 bits, see alignment E=1.2e-09 PF05175: MTS" amino acids 18 to 110 (93 residues), 39.4 bits, see alignment E=1.7e-13 PF13649: Methyltransf_25" amino acids 22 to 117 (96 residues), 32.6 bits, see alignment E=3.7e-11 PF08241: Methyltransf_11" amino acids 23 to 116 (94 residues), 24.1 bits, see alignment E=1.7e-08

Best Hits

Swiss-Prot: 100% identical to TRMN6_SHEON: tRNA1(Val) (adenine(37)-N6)-methyltransferase (SO_0948) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: None (inferred from 100% identity to son:SO_0948)

Predicted SEED Role

"tRNA (adenine37-N(6))-methyltransferase TrmN6 (EC 2.1.1.223)" (EC 2.1.1.223)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.223

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8EI95 at UniProt or InterPro

Protein Sequence (220 amino acids)

>SO0948 conserved hypothetical protein (NCBI ptt file) (Shewanella oneidensis MR-1)
MPVSTDGVILGAWSPLSQAQQILDIGAGSGLLSLMAAQRSLGRITAVELEEKAAAACLYN
MVQSPWADRLSVIHCDIQDFCQQTQYHGFFDHIICNPPYFEHGPQANDSHRAMARHTNTL
GFAPLLDAIIQCLTPQGYASLILPSQSMPRFKACLTSSKLHLTEEVLVKSVENKAANRVL
LLLSKNRQSDYRQSELTIRGIDGLYTKHMIELTRDFYLKL