Protein Info for SO0848 in Shewanella oneidensis MR-1

Name: napA
Annotation: periplasmic nitrate reductase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 826 signal peptide" amino acids 1 to 33 (33 residues), see Phobius details TIGR01706: periplasmic nitrate reductase, large subunit" amino acids 3 to 826 (824 residues), 1535.7 bits, see alignment E=0 PF10518: TAT_signal" amino acids 3 to 22 (20 residues), 25.5 bits, see alignment (E = 1.8e-09) TIGR01409: Tat (twin-arginine translocation) pathway signal sequence" amino acids 3 to 23 (21 residues), 17.5 bits, see alignment (E = 4.2e-07) PF04879: Molybdop_Fe4S4" amino acids 39 to 91 (53 residues), 70 bits, see alignment 2.6e-23 PF00384: Molybdopterin" amino acids 95 to 567 (473 residues), 222.7 bits, see alignment E=1.5e-69 PF01568: Molydop_binding" amino acids 712 to 820 (109 residues), 97.4 bits, see alignment E=1.1e-31

Best Hits

Swiss-Prot: 76% identical to NAPA_AERS4: Periplasmic nitrate reductase (napA) from Aeromonas salmonicida (strain A449)

KEGG orthology group: K02567, periplasmic nitrate reductase NapA [EC: 1.7.99.4] (inferred from 76% identity to asa:ASA_2771)

MetaCyc: 73% identical to periplasmic nitrate reductase subunit NapA (Escherichia coli K-12 substr. MG1655)
Nitrate reductase (cytochrome). [EC: 1.9.6.1]

Predicted SEED Role

"Periplasmic nitrate reductase precursor (EC 1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.7.99.4 or 1.9.6.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8EIJ1 at UniProt or InterPro

Protein Sequence (826 amino acids)

>SO0848 periplasmic nitrate reductase (NCBI ptt file) (Shewanella oneidensis MR-1)
MSISRREFLKANAAVAAATAVGVTLPVKMVEAAESDNIKWDKAPCRFCGVGCSVLVGTKA
GKVVATKGDPESPVNRGLNCIKGYFLSKIMYGKDRLTTPLLRMKDGKYHKEGEFTPVSWD
VAFDTMAAKWKHSIATKGPTSVGMFGSGQWTIWEGYAASKLHKAGFLTNNIDPNARHCMA
SAVVGFMRTFGIDEPMGCYDDLEAADHFVLWGANMAEMHPILWARLSDRRLSSKDCRVHV
LSTFENRSFDLADNPMVFHPQSDLVILNYIANYIIQNKAVNTDFVTKHTKFALGVDDIGY
GLRPDHPLEKKAKNPGNGKSTPISFDEYAKFVSTYTLEYAAKMSGVEPEKLETLAKAYAD
PKAKVMSLWTMGINQHVRGVWANNMLYNIHLLTGKIATPGNSPFSLTGQPSACGTAREVG
TFAHRLPADMVVDNDKHRAITEKMWQVPEGTIPPKPGYHAVLQSRMLKDGKLNCYWTMCT
NNMQAGPNINEEMYPGFRNPENFIVVSDPYPTVTAMAADLILPTAMWVEKEGAYGNAERR
THMWHQQVKAPEGAKSDLWQLVEFSKRFKVAEVWPAELIAKKPEYADKTLYEVLFANGVI
NKFPTTDCKGDLNDESEHFSFYVQKGIFEEYAAFGRGHGHDLAEFDRYHETRGLRWPVVN
DKETLRRFVEGSDPYVKAGEGYNFYGKPDGKAVIFALPYEPAAEEPNSEYDLWMSTGRVL
EHWHTGSMTARVPELYRAYPDAQIFMHPEDAKARGLQRGDEVLVASPRGEIKTRVETKGR
NKPPRGVVFMPFFDARQLVNKLILDATDPLSKETDFKKCPVKVMKA