Protein Info for SO0613 in Shewanella oneidensis MR-1

Name: pabA
Annotation: para-aminobenzoate synthase glutamine amidotransferase, component II (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00566: glutamine amidotransferase of anthranilate synthase or aminodeoxychorismate synthase
  • amino acids 1 to 188 (188 residues), 272 bits, E=1.4e-85
• PF00117: GATase
  • amino acids 3 to 188 (186 residues), 191.9 bits, E=9.3e-61
• PF07722: Peptidase_C26
  • amino acids 66 to 172 (107 residues), 35.5 bits, E=9.6e-13

Best Hits

Swiss-Prot: 66% identical to PABA_SERMA: Aminodeoxychorismate synthase component 2 (pabA) from Serratia marcescens

KEGG orthology group: K01664, para-aminobenzoate synthetase component II [EC: 2.6.1.85] (inferred from 100% identity to son:SO_0613)

MetaCyc: 65% identical to aminodeoxychorismate synthase subunit 2 (Escherichia coli K-12 substr. MG1655)
Aminodeoxychorismate synthase. [EC: 2.6.1.85]

Predicted SEED Role

"Para-aminobenzoate synthase, amidotransferase component (EC 2.6.1.85)" in subsystem Chorismate: Intermediate for synthesis of PAPA antibiotics, PABA, anthranilate, 3-hydroxyanthranilate and more. or Folate Biosynthesis or Tryptophan synthesis (EC 2.6.1.85)

MetaCyc Pathways

• superpathway of chorismate metabolism (50/59 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
• L-citrulline biosynthesis (7/8 steps found)
• L-glutamate and L-glutamine biosynthesis (6/7 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• superpathway of tetrahydrofolate biosynthesis (8/10 steps found)
• 4-aminobenzoate biosynthesis I (2/2 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (1/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (1/4 steps found)
• chloramphenicol biosynthesis (1/9 steps found)
• superpathway of candicidin biosynthesis (2/11 steps found)

KEGG Metabolic Maps

• Biosynthesis of phenylpropanoids
• Folate biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.85

Use Curated BLAST to search for 2.6.1.85

Sequence Analysis Tools

See Q8EJ59 at UniProt or InterPro

Protein Sequence (191 amino acids)

>SO0613 para-aminobenzoate synthase glutamine amidotransferase, component II (NCBI ptt file) (Shewanella oneidensis MR-1)
MLLMIDNYDSFTFNLVQYFQQLGQEIVVKRNDEISLEGIEALAPSHLVISPGPCSPNEAG
ISLAAIEHFATRLPILGVCLGHQAMAQVFGAKVVRAQRVMHGKVSAIAHTGERLFKGLNQ
PLTVTRYHSLLVDTVPKDFVLDAWFDDPTHGREIMAMSHKELPLFGVQFHPESILTEQGH
ELLANFLSQST