Protein Info for SO0604 in Shewanella oneidensis MR-1

Name: hflX
Annotation: GTP-binding protein HflX (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03156: GTP-binding protein HflX
  • amino acids 11 to 359 (349 residues), 510.5 bits, E=1e-157
• PF13167: GTP-bdg_N
  • amino acids 25 to 112 (88 residues), 110.1 bits, E=1.3e-35
• PF16360: GTP-bdg_M
  • amino acids 114 to 192 (79 residues), 105.3 bits, E=4.1e-34
• PF01926: MMR_HSR1
  • amino acids 200 to 317 (118 residues), 76.2 bits, E=4.5e-25
• PF00025: Arf
  • amino acids 201 to 325 (125 residues), 25.1 bits, E=2.2e-09

Best Hits

Swiss-Prot: 64% identical to HFLX_ECOLI: GTPase HflX (hflX) from Escherichia coli (strain K12)

KEGG orthology group: K03665, GTP-binding protein HflX (inferred from 100% identity to son:SO_0604)

MetaCyc: 64% identical to ribosome rescue factor HflX (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"GTP-binding protein HflX" in subsystem Hfl operon or Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Sequence Analysis Tools

See Q8EJ68 at UniProt or InterPro

Protein Sequence (435 amino acids)

>SO0604 GTP-binding protein HflX (NCBI ptt file) (Shewanella oneidensis MR-1)
MFDRYEAGETAVLVHIDFSDEERREDLVELQLLVESAGARSVGVITGSRRSPDRKFFIGS
GKAEELAALVAATEANVVIFNHALSPAQERNLEQVCQCRVLDRTSLILDIFAQRARTHEG
KLQVELAQLRHMSTRLVRGWTHLERQKGGIGLRGPGETQLETDRRLLRGRIKNINKRLER
VDKQREQSRRARKRSDMPTVSLVGYTNAGKSTLFNALTSSDVYAADQLFATLDPTLRKLD
LPDGAVILADTVGFIRHLPHDLVAAFKATLQETRQAELLLHIVDCADENMADNFEQVQSV
LKEIDADEVMQLIVCNKIDLLEDVTPRIEYDDRGKPVRVWVSAHKRLGFDLLLKAITELI
GEVIHEFTLRIPATAGHYLGQFYRLDAIQQKEYDDLGNCILSVRLSDADWRRLAKQSQGE
LETFIFDPSIEKAVC