Protein Info for SO0600 in Shewanella oneidensis MR-1

Name: amiB
Annotation: N-acetylmuramoyl-L-alanine amidase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF11741: AMIN
  • amino acids 32 to 132 (101 residues), 63.2 bits, E=3.1e-21
• PF01520: Amidase_3
  • amino acids 165 to 384 (220 residues), 158 bits, E=3.8e-50
• PF01476: LysM
  • amino acids 419 to 461 (43 residues), 48.7 bits, 8.7e-17

Best Hits

KEGG orthology group: K01448, N-acetylmuramoyl-L-alanine amidase [EC: 3.5.1.28] (inferred from 100% identity to son:SO_0600)

Predicted SEED Role

"N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)" (EC 3.5.1.28)

MetaCyc Pathways

• peptidoglycan recycling I (9/14 steps found)
• peptidoglycan recycling II (4/10 steps found)

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.28

Use Curated BLAST to search for 3.5.1.28

Sequence Analysis Tools

See Q8EJ71 at UniProt or InterPro

Protein Sequence (463 amino acids)

>SO0600 N-acetylmuramoyl-L-alanine amidase (NCBI ptt file) (Shewanella oneidensis MR-1)
MTKNKHYFQGIILLLCTFFAVAAQAANQLESVRIWAAPESTRIVFDLSEVPNYSYFTLDG
PNRLVVDLKKASTKLNLKNVDNNSKLVKGIRVSKSPTKGDLRLVIDLVKPLNPNLFSLPV
TAPYGNRLVVDLEDKSATAELSAVSSTPVKKNVTQSVQASRDIVIAIDAGHGGDDPGSIG
PSGLYEKKVVFEISKRLASKINDTPGMRAVMIRSGDYFVNLNRRSELARNSKADLLISIH
ADAFTSPNPRGASVWVLSMRRANSEIGRWLEQKEKHSELLGGAGEIIQNTDNEQYLAMTL
LDMSMNSSMAIGHSVAGDILKDLGAVTELHKSRPESASFAVLKSPDIPSILVETGFISNP
KEERLLSSSRHQESIATAIYKGVNRYFHNNPPADTLLAKKNTTGSTVKSSSKSAGTIKHK
VSRGESLSAIAQRYKVPMASIIRANGMKTDVVQLGQTLVIPES