Protein Info for SO0432 in Shewanella oneidensis MR-1

Name: acnB
Annotation: aconitate hydratase 2 (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00117: aconitate hydratase 2
  • amino acids 1 to 853 (853 residues), 1571 bits, E=0
• PF11791: Aconitase_B_N
  • amino acids 4 to 156 (153 residues), 216.8 bits, E=2.2e-68
• PF06434: Aconitase_2_N
  • amino acids 168 to 382 (215 residues), 331.9 bits, E=2.2e-103
• PF00330: Aconitase
  • amino acids 471 to 818 (348 residues), 139.7 bits, E=2.2e-44

Best Hits

Swiss-Prot: 81% identical to ACNB_ECOLI: Aconitate hydratase B (acnB) from Escherichia coli (strain K12)

KEGG orthology group: K01682, aconitate hydratase 2 [EC: 4.2.1.3] (inferred from 83% identity to aha:AHA_3856)

MetaCyc: 80% identical to aconitate hydratase 2 (Salmonella enterica enterica serovar Typhimurium)
2-methylisocitrate dehydratase. [EC: 4.2.1.99]; Aconitate hydratase. [EC: 4.2.1.99, 4.2.1.3]

Predicted SEED Role

"Aconitate hydratase 2 (EC 4.2.1.3) @ 2-methylisocitrate dehydratase (EC 4.2.1.99)" (EC 4.2.1.3, EC 4.2.1.99)

MetaCyc Pathways

• mixed acid fermentation (15/16 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
• TCA cycle III (animals) (9/10 steps found)
• glyoxylate cycle (6/6 steps found)
• TCA cycle II (plants and fungi) (8/9 steps found)
• 2-methylcitrate cycle I (5/5 steps found)
• superpathway of glyoxylate bypass and TCA (10/12 steps found)
• nitrogen remobilization from senescing leaves (7/8 steps found)
• ethene biosynthesis V (engineered) (19/25 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• TCA cycle I (prokaryotic) (8/10 steps found)
• 2-methylcitrate cycle II (5/6 steps found)
• TCA cycle V (2-oxoglutarate synthase) (7/9 steps found)
• TCA cycle VI (Helicobacter) (7/9 steps found)
• partial TCA cycle (obligate autotrophs) (6/8 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (6/9 steps found)
• reductive TCA cycle I (7/11 steps found)
• TCA cycle VII (acetate-producers) (5/9 steps found)
• reductive TCA cycle II (6/12 steps found)
• methylaspartate cycle (9/19 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glyoxylate and dicarboxylate metabolism
• Propanoate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.3, 4.2.1.99

Use Curated BLAST to search for 4.2.1.3 or 4.2.1.99

Sequence Analysis Tools

See Q8EJN1 at UniProt or InterPro

Protein Sequence (865 amino acids)

>SO0432 aconitate hydratase 2 (NCBI ptt file) (Shewanella oneidensis MR-1)
MLEAYRKHVAERAAEGVVPKPLDAHQVAELVKLVQNPPAGEEAFVLDLLENRIPPGVDEA
AYVKAAFLDAVAKGTATSPILSAERATELLGTMQGGYNIEPLIAQLDNPALAPLAAKALA
HTLLMFDSFHDVVEKMEAGNQFAKQVVEAWANADWYLSRPKLADKVTLTVFKVSGETNTD
DLSPAPDAWSRPDIPLHALAMLKNARDGIEPDVAGSVGPIKKLEELKTKGFPLVYVGDVV
GTGSSRKSATNSVLWFMGDDIPFVPNKRAGGFCLGGKIAPIFFNTMEDAGALPIELDVSK
MEMGDVIDIYPYAGVVKRHGSDEVISEFSLKTDVLLDEVRAGGRIPLIIGRGLTDKARAV
LGLPASEVFVRPQDIADNGKGYTLAQKMVGKACGVAGVRPGQYCEPKMTSVGSQDTTGPM
TRDELKDLACLGFSADLTMQSFCHTAAYPKPVDVNTHHTLPDFIMNRGGVSLRPGDGVIH
SWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGKM
QPGITLRDLVHAIPLKAIELGMLTVEKKGKVNIFSGRVLEIEGLESLKVEQAFELSDASA
ERSAAGCTIKLDKEPIIEYLNSNITMLKWMIAEGYGDRRTIERRIKGMEEWLANPELMSA
DADAEYTAVIEIDLNEIKEPILCAPNDPDDAVLLSSVAQTKIDEVFVGSCMTNIGHFRAT
GKMLDKFAKTLPTRLWIAPPTKMDKDQLTEEGYYGIFGRVGARIEIPGCSLCMGNQARVA
EGATVVSTSTRNFPNRLGTGANVYLASAELAAVAALLGRLPTVEEYQEYAKELDATAADT
YRYLNFDQIDSYTKKASQVIFQSAV