Protein Info for SO0357 in Shewanella oneidensis MR-1

Annotation: metallo-beta-lactamase superfamily protein (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 617 PF00753: Lactamase_B" amino acids 85 to 159 (75 residues), 55.1 bits, see alignment E=2.6e-18 PF14863: Alkyl_sulf_dimr" amino acids 348 to 486 (139 residues), 195.7 bits, see alignment E=1.2e-61 PF14864: Alkyl_sulf_C" amino acids 495 to 617 (123 residues), 145.8 bits, see alignment E=2e-46 PF02036: SCP2" amino acids 528 to 605 (78 residues), 28.7 bits, see alignment E=3.6e-10

Best Hits

Swiss-Prot: 48% identical to YJCS_ECOLI: Putative alkyl/aryl-sulfatase YjcS (yjcS) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to son:SO_0357)

MetaCyc: 48% identical to linear primary-alkylsulfatase (Escherichia coli K-12 substr. MG1655)
RXN-15761 [EC: 3.1.6.21]

Predicted SEED Role

"alkyl sulfatase (EC 3.1.6.-)" (EC 3.1.6.-)

Isozymes

Compare fitness of predicted isozymes for: 3.1.6.-

Use Curated BLAST to search for 3.1.6.- or 3.1.6.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (617 amino acids)

>SO0357 metallo-beta-lactamase superfamily protein (NCBI ptt file) (Shewanella oneidensis MR-1)
MQKTLPFNNKQDFEDAQRGFIAKPETLTIKDDKGNVVWDLEQYKTYIGLDKVAPNTVNPS
LWRNAQLNMQYGLFKVTDKIYQIRGFDLSNITFIEGEKGWVVFDPLISPETAKAALAFIN
KTLGERPVTAVVYSHSHVDHYGGAAGLFASPDEVLRNKVEIIAPEGFTEHAVSENVIAGN
AMARRAIYMYGALLPRNPQGGVNGGLGQTTSTGIPSLLLPTKLIEKTEEEVTVDGVRMVF
QLTPGTEAPAEMNTWFPDSKALWMAENTTSTMHNILTLRGAQVRDSLKWASFLNETIEIW
GSQAEVKFQSHHWPRWGNTYIVDYFKKQRDLYKYIHDQSVRLMNMGYTGEEISEQIVLPP
ELNDFWPNRGYYGTLRHNSRAVYQRYMGWYSGNPSDLDNLPPEMVGPKYIEFMGGEKEVL
IKAKASFDKGEYRWVAEVLKHLVFANPDNKEGKLLLADALEQLGYQAESGPWRSVYLQGA
YELRNGVPSGGGTVTATPDTIRAMTPTMLFEYLSVRINPEKAAGKKMIINMNFTDIGEKH
TLSLENAVLNHTTRYATKPDVTITLSKKVMDDIQLGQVTLEQKIASGDIKIEGDQQKFND
FFSMLDKFNFWFNIVTP