Protein Info for SO0343 in Shewanella oneidensis MR-1

Name: acnA
Annotation: aconitate hydratase 1 (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02333: 2-methylisocitrate dehydratase, Fe/S-dependent
  • amino acids 6 to 863 (858 residues), 1873 bits, E=0
• PF00330: Aconitase
  • amino acids 70 to 537 (468 residues), 453.9 bits, E=7.4e-140
• PF00694: Aconitase_C
  • amino acids 661 to 792 (132 residues), 97.7 bits, E=7e-32

Best Hits

Swiss-Prot: 100% identical to ACND_SHEON: 2-methylcitrate dehydratase (2-methyl-trans-aconitate forming) (acnD) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K01681, aconitate hydratase 1 [EC: 4.2.1.3] (inferred from 85% identity to bam:Bamb_4840)

MetaCyc: 100% identical to 2-methylcitrate dehydratase (Shewanella oneidensis)
RXN-8979 [EC: 4.2.1.117]

Predicted SEED Role

"2-methylcitrate dehydratase FeS dependent (EC 4.2.1.79)" in subsystem Methylcitrate cycle or Propionate-CoA to Succinate Module (EC 4.2.1.79)

MetaCyc Pathways

• mixed acid fermentation (15/16 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
• TCA cycle III (animals) (9/10 steps found)
• glyoxylate cycle (6/6 steps found)
• TCA cycle II (plants and fungi) (8/9 steps found)
• 2-methylcitrate cycle I (5/5 steps found)
• superpathway of glyoxylate bypass and TCA (10/12 steps found)
• nitrogen remobilization from senescing leaves (7/8 steps found)
• ethene biosynthesis V (engineered) (19/25 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• TCA cycle I (prokaryotic) (8/10 steps found)
• 2-methylcitrate cycle II (5/6 steps found)
• TCA cycle V (2-oxoglutarate synthase) (7/9 steps found)
• TCA cycle VI (Helicobacter) (7/9 steps found)
• partial TCA cycle (obligate autotrophs) (6/8 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (6/9 steps found)
• reductive TCA cycle I (7/11 steps found)
• TCA cycle VII (acetate-producers) (5/9 steps found)
• reductive TCA cycle II (6/12 steps found)
• methylaspartate cycle (9/19 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glyoxylate and dicarboxylate metabolism
• Propanoate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.3

Use Curated BLAST to search for 4.2.1.117 or 4.2.1.3 or 4.2.1.79

Sequence Analysis Tools

See Q8EJW3 at UniProt or InterPro

Protein Sequence (867 amino acids)

>SO0343 aconitate hydratase 1 (NCBI ptt file) (Shewanella oneidensis MR-1)
MSTVMNTQYRKPLPGTALDYFDTREAIEAIAPGAYAKLPYTSRVLAENLVRRCEPEMLTA
SLKQIIESKQELDFPWFPARVVCHDILGQTALVDLAGLRDAIAAKGGDPAQVNPVVPTQL
IVDHSLAVEYGGFDKDAFAKNRAIEDRRNEDRFHFINWTQKAFKNIDVIPQGNGIMHQIN
LERMSPVIHARNGVAFPDTLVGTDSHTPHVDALGVIAIGVGGLEAESVMLGRASYMRLPD
IIGVELTGKPQPGITATDIVLALTEFLRAQKVVSSYLEFFGEGAEALTLGDRATISNMTP
EFGATAAMFYIDQQTLDYLTLTGREAEQVKLVETYAKTAGLWSDDLKQAVYPRTLHFDLS
SVVRTIAGPSNPHARVPTSELAARGISGEVENEPGLMPDGAVIIAAITSCTNTSNPRNVI
AAGLLARNANAKGLTRKPWVKTSLAPGSKAVQLYLEEANLLPELESLGFGIVGFACTTCN
GMSGALDPVIQQEVIDRDLYATAVLSGNRNFDGRIHPYAKQAFLASPPLVVAYAIAGTIR
FDIEKDVLGLDKDGKPVRLINIWPSDAEIDAVIAASVKPEQFRKVYEPMFDLSVDYGDKV
SPLYDWRPQSTYIRRPPYWEGALAGERTLKGMRPLAVLGDNITTDHLSPSNAIMMDSAAG
EYLHKMGLPEEDFNSYATHRGDHLTAQRATFANPKLKNEMAIVDGKVKQGSLARIEPEGI
VTRMWEAIETYMDRKQPLIIIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNL
VGMGVLPLEFKAGENRATYGIDGTEVFDVIGSIAPRADLTVIITRKNGERVEVPVTCRLD
TAEEVSIYEAGGVLQRFAQDFLESNLK