Protein Info for SO0342 in Shewanella oneidensis MR-1

Annotation: conserved hypothetical protein (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 397 transmembrane" amino acids 324 to 335 (12 residues), see Phobius details TIGR02334: probable AcnD-accessory protein PrpF" amino acids 8 to 396 (389 residues), 761.2 bits, see alignment E=9.3e-234 PF04303: PrpF" amino acids 9 to 395 (387 residues), 585.5 bits, see alignment E=2.1e-180

Best Hits

Swiss-Prot: 100% identical to PRPF_SHEON: 2-methyl-aconitate isomerase (prpF) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K09788, hypothetical protein (inferred from 100% identity to son:SO_0342)

Predicted SEED Role

"2-methylaconitate cis-trans isomerase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8EJW4 at UniProt or InterPro

Protein Sequence (397 amino acids)

>SO0342 conserved hypothetical protein (NCBI ptt file) (Shewanella oneidensis MR-1)
MSNKLFPPQIKVAATYMRGGTSKGVFFRLQDLPEAAQVPGPARDALLLRVIGSPDPYAKQ
IDGMGGATSSTSKTVILSHSSKANHDVDYLFGQVSIDKPFVDWSGNCGNLTAAVGAFAIS
NGLIDAARIPRNGVCTVRIWQANIGKTIIAHVPITDGAVQETGDFELDGVTFPAAEVQIE
FMNPAADDDGEGGCMFPTGNLVDVLEVPGIGRFNATMINAGIPTIFINAEDLGYTGTELQ
DDINSDNAALAKFETIRAHGALRMGLIKHIDEAASRQHTPKIAFVAPPKSYASSSGKTVA
AEDVDLLVRALSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGGEKEAVRFGHPSGTL
RVGAQAVQENGEWTVIKAIMSRSARVLMEGFVRVPKP