Protein Info for SO0333 in Shewanella oneidensis MR-1

Name: dsbA
Annotation: thiol:disulfide interchange protein DsbA (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 202 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF13462: Thioredoxin_4" amino acids 35 to 194 (160 residues), 52.3 bits, see alignment E=7.5e-18 PF01323: DSBA" amino acids 43 to 184 (142 residues), 68.1 bits, see alignment E=9.5e-23

Best Hits

Swiss-Prot: 38% identical to DSBA_VIBCH: Thiol:disulfide interchange protein DsbA (dsbA) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K03673, thiol:disulfide interchange protein DsbA (inferred from 100% identity to son:SO_0333)

Predicted SEED Role

"Periplasmic thiol:disulfide interchange protein DsbA" in subsystem Biogenesis of c-type cytochromes or Periplasmic disulfide interchange

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8EJX3 at UniProt or InterPro

Protein Sequence (202 amino acids)

>SO0333 thiol:disulfide interchange protein DsbA (NCBI ptt file) (Shewanella oneidensis MR-1)
MKKALLMAAALLLAPLTVSAAEYKEGVHYTVINDGPATAKPEITEFFSFYCPHCYTFSKT
VVPKILAEKPAGVEFNQAHVDFIGKEMGIEMSRAFAVAHQLNVNEKIDTALFTAIHDKKQ
HFTSRDDVRALFVANGVDGKTFDAAADSFMVKAQMSKMKRDTENAKLTGVPALVVNGKYR
VETGAIKSYDELLDIAYYLARK