Protein Info for SO0101 in Shewanella oneidensis MR-1
Name: fdnG
Annotation: selenium-containing formate dehydrogenase, nitrate inducible, alpha subunit (NCBI ptt file)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 63% identical to FDNG_ECOLI: Formate dehydrogenase, nitrate-inducible, major subunit (fdnG) from Escherichia coli (strain K12)
KEGG orthology group: K08348, formate dehydrogenase-N, alpha subunit [EC: 1.2.1.2] (inferred from 63% identity to cro:ROD_15621)MetaCyc: 60% identical to formate dehydrogenase O subunit alpha (Escherichia coli K-12 substr. MG1655)
FORMATEDEHYDROG-RXN [EC: 1.17.5.3]
Predicted SEED Role
"Formate dehydrogenase N alpha subunit (EC 1.2.1.2) @ selenocysteine-containing" (EC 1.2.1.2)
MetaCyc Pathways
- formate to dimethyl sulfoxide electron transfer (2/2 steps found)
- formate to nitrite electron transfer (2/3 steps found)
- formate to trimethylamine N-oxide electron transfer (1/2 steps found)
- nitrate reduction III (dissimilatory) (1/2 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.2.1.2
Use Curated BLAST to search for 1.17.5.3 or 1.2.1.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q8EKJ1 at UniProt or InterPro
Protein Sequence (1004 amino acids)
>SO0101 selenium-containing formate dehydrogenase, nitrate inducible, alpha subunit (NCBI ptt file) (Shewanella oneidensis MR-1) MNRRQFFKLCAAGAATSAISALGLMSEKAYAAVREFKLLGAKETRNNCPYCSVGCGLLMY SQGSGGKNSEHAIFHIEGDADHPVNRGALCSKGAGLVDYVNSPHRLQYPEYRAPGSNKWE RISWQDAFKRIARLMKDDRDANLIEKNADGVTVNRWLTTGMMTSSGMANESGLATQKFAR ALGLVAIDTIARNUHSPTVASLAPTFGRGAMTNHWIDIKNSNVVIIMGGNAAEAHPVGFG WVTEAMQHNNAKLIVVDPRFNRSASLADHYAPIRSGTDIAFLLGVIRYLISTNQVNFDYV KAYTNASYLVRDDFDFHDGLFSGFDEAKGEYNKESWFYQLDEDGYAIVDETLEHPRCVWN LLKQHVERYDFATVSNITGTPTEDYQVVCDAIASTHTKDRVATFMYALGWTHHSKGAQNI RSMAMIQLLLGNIGQLGGGVNALRGHANVQGSTDMGLLAQSLPGYLKLPNDKEPTLAAHL AANTPKPLRPGQTNYWQNYPKFYVSLLKAFWGENATPENEFGYQWLPKWDQMYDFGKHLD MMYRGKVNGCIVQGVNAINSMPNRNKNIKALSNLKFLVVLDNLSSETATFWQNEPGFNEV DTASIQTEVFRLPATVFAEEEGSIVNSGRWMQWHYKWANPPGEAMSDSEIVSGLLLELRK LYREEGGKLPEPIQAINWNYTDPHNPSSIELTKELNGYDVATKRQISSFAELKADGSTAS ACWVYAGSWTEAGNQMARRDNHDPSGKGITPGWAFAWPLNRRVLYNRASCDVNGKPWDEH RKIVEWKDGKWEGIDVPDFNAKLNPQESAHPFIMQADGVGRFFALKLLKEGPFPEHYEPV ESPIGTNPLHPNVVHSPVLRWFEGVKDTIGTKEEYPYACTTYSLTEHFNFWTTHCRLAAI AMPETFVEMNEQLAAEKGIKNGDWVKVSSKRGHILTKALVTKRMRPLQVNGQTVHTLGIP RHGSHNALTRKSYSCNVLTTEMGDANTGVPEYKAFLVNVEKAEV