Protein Info for Rru_A3695 in Rhodospirillum rubrum S1H

Annotation: Acetoacetyl-CoA synthase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 668 transmembrane" amino acids 336 to 352 (17 residues), see Phobius details TIGR01217: acetoacetate-CoA ligase" amino acids 20 to 665 (646 residues), 934.3 bits, see alignment E=1.6e-285 PF16177: ACAS_N" amino acids 52 to 107 (56 residues), 34.5 bits, see alignment 2.2e-12 PF00501: AMP-binding" amino acids 114 to 486 (373 residues), 154.9 bits, see alignment E=4.2e-49 PF13193: AMP-binding_C" amino acids 560 to 630 (71 residues), 34.7 bits, see alignment E=4.2e-12

Best Hits

Swiss-Prot: 64% identical to AACS_RHIME: Acetoacetyl-coenzyme A synthetase (acsA2) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K01907, acetoacetyl-CoA synthetase [EC: 6.2.1.16] (inferred from 100% identity to rru:Rru_A3695)

Predicted SEED Role

"Acetoacetyl-CoA synthetase (EC 6.2.1.16)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism or Polyhydroxybutyrate metabolism (EC 6.2.1.16)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.2.1.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RN06 at UniProt or InterPro

Protein Sequence (668 amino acids)

>Rru_A3695 Acetoacetyl-CoA synthase (NCBI) (Rhodospirillum rubrum S1H)
MTESEQAVISGFAKGEPAPPLWTPSAERIAATRLDAFRRRIEERHGVVLADYDALWQWSI
DAPAAFWTEVWDFCGVIGDKGDGPVVDDPGKLPGARWFAGARLNAAETLLAPHAQRPGDA
VVFRVENHPTQRLDLPTLRARVSLVQQGLRRAGVGVGDRVAGYLPNMPEALIAMLATAGL
GAVWSSASPDFGAQGVIDRFGQIEPKVLFTVDGYFYNGKVHDCREKVATLVKAVPSITQV
VEVAFAGKGDAPAAIPRATSWAAFIGDFTAGEPDFLRLPFDHPLYILYSSGTTGVPKCIV
HGHGGTLLQQLKEHVLHADAHPGDRVFYFTTCGWMMWNWLAAALGAGCTVLLYDGSPFYP
DGAVLWDYAQAEGCTFFGTSAKWIDAQRKARLRPSDTHDLSTVRVIASTGSPLAPESFDY
VYDAIKGDVQLASISGGTDILSCFMLGSPIDSVFRGEIQKRGLGMAVAVFDDEGRPLVGQ
KGELVCTRAFPSSPIGFWNDADGAKFKAAYFERFPGVWTHGDWVELTPHGGIVVYGRSDA
TLNPGGVRIGTAEIYRQVETLDEVREAIVIGQDWQGDVRVVLFVVLRDGLTLTPELEDKI
KARIRQSCTPRHVPAKVVQVNDIPRTKSNKIVELAVREVVHGRPVRNREALANPEALDDF
AGRPELAG