Protein Info for Rru_A3543 in Rhodospirillum rubrum S1H

Annotation: Periplasmic Sensor Signal Transduction Histidine Kinase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 478 transmembrane" amino acids 12 to 32 (21 residues), see Phobius details amino acids 38 to 59 (22 residues), see Phobius details PF13188: PAS_8" amino acids 120 to 166 (47 residues), 21.5 bits, see alignment 3.4e-08 PF08448: PAS_4" amino acids 123 to 226 (104 residues), 25.5 bits, see alignment E=2.7e-09 PF00512: HisKA" amino acids 231 to 295 (65 residues), 73.2 bits, see alignment E=2.9e-24 PF02518: HATPase_c" amino acids 343 to 466 (124 residues), 95.6 bits, see alignment E=5.3e-31

Best Hits

KEGG orthology group: K07636, two-component system, OmpR family, phosphate regulon sensor histidine kinase PhoR [EC: 2.7.13.3] (inferred from 100% identity to rru:Rru_A3543)

Predicted SEED Role

"Phosphate regulon sensor protein PhoR (SphS) (EC 2.7.13.3)" in subsystem High affinity phosphate transporter and control of PHO regulon or Phosphate metabolism (EC 2.7.13.3)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RNF8 at UniProt or InterPro

Protein Sequence (478 amino acids)

>Rru_A3543 Periplasmic Sensor Signal Transduction Histidine Kinase (NCBI) (Rhodospirillum rubrum S1H)
MDRLTPSLGRLIGAAMLVCVPATAVLLGLTLWGRLSVGVALVAGVLIWTLTALLLRSILR
EFMVVSRHIRGLGGRSEAGEGAAPTPRLEIAQGLLGAAQHLDRGWRRRTQALRDQLRSDE
QVLNTLPDPLLMIAKEAVVTRANEAARSLFGRDPAGQEIITLLRDPGVLDSVERVLAGGT
APRQTIWVMPGAVEREFEVRATPLPSPSIDGSVALLGLHDITALRRLEQMRADFVANASH
ELRTPLSSLIGFTETLSTSAKNDPEARERFLAIMLEQGRRMQRLIEDLLSLSRIEMEEHT
PPDEAVVLPGLIASVTRFLEIKAREKTMTLSVEAPPDLPPAIGDADQLSQIFQNLLDNAI
KYGRPGTPVEVRLAVVERGPPAMPAGLRAPCLRVSVIDHGEGIAKAHLPRLTERFYRVDK
ARSRALGGTGLGLAIVKHVVVRHRGAMTIESDPGLGTTVSVFLPLHGPRPPRRAAPSA