Protein Info for Rru_A3542 in Rhodospirillum rubrum S1H

Annotation: malate dehydrogenase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00390: malic
  • amino acids 23 to 155 (133 residues), 99.8 bits, E=2.5e-32
• PF03949: Malic_M
  • amino acids 169 to 363 (195 residues), 82.5 bits, E=4.7e-27
• PF01515: PTA_PTB
  • amino acids 435 to 748 (314 residues), 265.3 bits, E=1.3e-82

Best Hits

Swiss-Prot: 64% identical to MAO2_RHIME: NADP-dependent malic enzyme (tme) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K00029, malate dehydrogenase (oxaloacetate-decarboxylating)(NADP+) [EC: 1.1.1.40] (inferred from 100% identity to rru:Rru_A3542)

Predicted SEED Role

"NADP-dependent malic enzyme (EC 1.1.1.40)" in subsystem Pyruvate metabolism I: anaplerotic reactions, PEP (EC 1.1.1.40)

MetaCyc Pathways

• gluconeogenesis I (10/13 steps found)
• C4 photosynthetic carbon assimilation cycle, NADP-ME type (4/7 steps found)
• methylgallate degradation (2/6 steps found)
• protocatechuate degradation I (meta-cleavage pathway) (3/8 steps found)
• C4 photosynthetic carbon assimilation cycle, PEPCK type (7/14 steps found)
• superpathway of vanillin and vanillate degradation (3/10 steps found)
• syringate degradation (3/12 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of terpenoids and steroids
• Carbon fixation in photosynthetic organisms
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.40

Sequence Analysis Tools

See Q2RNF9 at UniProt or InterPro

Protein Sequence (753 amino acids)

>Rru_A3542 malate dehydrogenase (NCBI) (Rhodospirillum rubrum S1H)
MADETNRNLDREALLFHITGRPGKIEIAATKPLTTQRDLSLAYSPGVAAPCLAIQRDPTT
AYDYTAKGNMVAVISNGTAVLGLGDLGALASKPVMEGKAVLFKKFADVDGIDLEVDTRDV
DEFVNCVRYLGPTFGGINLEDIKAPECFIIEQRLRALMDIPVFHDDQHGTAIIAAAGLIN
ALDLSGRDIGDAKMVVNGAGAAAIACVELVKAMGMRSANVTLCDSKGVVHRGRSDLNQWK
SAHAIDTEARSLQDAMRGADVFLGLSVKGAVTAEMVATMAPQPIIFAMANPDPEITPEDV
LSVRDDAIIATGRSDYPNQINNVLGFPYIFRGALDVRARTINDEMKIAAARAIAALARED
VPDEVAAAYSGRRLRYGRDYIIPVPFDPRLIAAISPAVAEAAMRSGVARKPIIDIIGYRR
ELSARLDPTAASLQALSAQVINNPKRVAFAEGEEEKMIRAALSYRNAGYGTPILIGRAEK
IAATMAAMGLGGLEGMEIQNAKLSTQTKPYTDYLYGRLQRHGYLYRDVQRLVNSDRNVFA
ASMVMLQDADAMVTGTIRHFHSCLSDITKVIDQTPGELVFSVTLLIARGRTVFIADTNVH
EAPSAQELADIAIQTAALARRMGHEPRVALLSYSTFGAPLGRTPDRPRQAVALLEDRKVE
FEFDGEIGVHVALDPDLLKLYPFCRLSAPANVLIMPGLHSANISSKLMQKMGGATTIGPV
LMGLSRPCQIVPMDATVSDIVHMATLAAAEAGR