Protein Info for Rru_A3514 in Rhodospirillum rubrum S1H

Annotation: DNA polymerase A (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 924 PF02739: 5_3_exonuc_N" amino acids 7 to 168 (162 residues), 192.6 bits, see alignment E=8e-61 TIGR00593: DNA polymerase I" amino acids 7 to 924 (918 residues), 912.8 bits, see alignment E=1.5e-278 PF01367: 5_3_exonuc" amino acids 169 to 264 (96 residues), 102.9 bits, see alignment E=2.4e-33 PF01612: DNA_pol_A_exo1" amino acids 306 to 504 (199 residues), 114.9 bits, see alignment E=7.6e-37 PF00476: DNA_pol_A" amino acids 538 to 922 (385 residues), 504.7 bits, see alignment E=3.3e-155

Best Hits

KEGG orthology group: K02335, DNA polymerase I [EC: 2.7.7.7] (inferred from 100% identity to rru:Rru_A3514)

Predicted SEED Role

"DNA polymerase I (EC 2.7.7.7)" in subsystem DNA-replication or DNA Repair Base Excision (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RNI7 at UniProt or InterPro

Protein Sequence (924 amino acids)

>Rru_A3514 DNA polymerase A (NCBI) (Rhodospirillum rubrum S1H)
MTEPKHLYLVDGSGYIFRAYHSLPPMTRTDGTPVNAVYGFTAMLMKLLADMDADHLAVIF
DKARVSFRNEIYPLYKAQRPPPPEDLIPQFPLIREAVRAFNVPCIERDGFEADDLIATYA
KRARAEGAIVTVVSSDKDLMQLVGEGVALFDPMKNRAIGPAEVFEKFGVAPDKVIDVQAL
AGDAVDNVPGVPGIGVKTAAQLIGEYGDLETLLARAAEIKQPKRRQSLLDHAEAARISLQ
LVRLRDDVEVSETLADFATAEPDAEVLAGFLAENGFRSLLARVTAVAHAKAGTTPQPAAA
PADDDYVLVNTLAELGVWIAKARAAGLVAIDTETDSLKARRATLVGVSLAITPGRACYIP
FAHGLRKAEGLDFDGAAPPVANIEDKAAALALLGDLLRDPGVLKVGHNLKFDLHVLANAG
LSDVAPVDDTMVLSYVLDGTLHGHGMDELAGLHLGRRTITFEEVCGKGKGQITFDQVPLD
KAVAYAAEDADVTLRLYLLLRDRLLKEHMVRVHETLDRPMVRILTDMEAAGVRVDALRLK
ALSGDFAKRMGDLEDQIHALAGHAFNVASPKQLGEVLFEEMSLTGGKKSRKTGAWSTDAQ
VLDQLAAEGHDLPARVLDWRQYAKLKGTYTDALVAEIDERTGRVHTTYGLTVTATGRLSS
NDPNLQNIPVRSEAGRKIREAFIAAPGHLLISADYSQIELRLVAHVAGITALRQAFAEGK
DIHAITASQVFGVPVEGMDPMVRRQAKAINFGIIYGISAHGLAQQLGVGRPEAAAFITAY
FARFPEIRSYMETVKEEARTVGWVATPFGRRIPIPGIQDKNPAARAFAERQAINAPIQGG
AADIIKRAMIALPPALEQAGLATRMLLQVHDELIFEAPEAEAETAAALVKRVMETAVTLT
DDQGERTVPLEVDTGIAASWAEAH