Protein Info for Rru_A3400 in Rhodospirillum rubrum S1H

Annotation: Thiosulphate-binding protein (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 354 signal peptide" amino acids 1 to 40 (40 residues), see Phobius details TIGR00971: sulfate ABC transporter, sulfate-binding protein" amino acids 39 to 349 (311 residues), 472.4 bits, see alignment E=3.4e-146 PF13416: SBP_bac_8" amino acids 51 to 319 (269 residues), 40.6 bits, see alignment E=5.4e-14 PF01547: SBP_bac_1" amino acids 52 to 293 (242 residues), 53.6 bits, see alignment E=7.3e-18 PF13531: SBP_bac_11" amino acids 57 to 301 (245 residues), 131 bits, see alignment E=1.2e-41 PF13343: SBP_bac_6" amino acids 150 to 312 (163 residues), 25.7 bits, see alignment E=1.6e-09

Best Hits

Swiss-Prot: 66% identical to SUBI_SHIFL: Sulfate-binding protein (sbp) from Shigella flexneri

KEGG orthology group: K02048, sulfate transport system substrate-binding protein (inferred from 100% identity to rru:Rru_A3400)

MetaCyc: 66% identical to sulfate/thiosulfate ABC transporter periplasmic binding protein Sbp (Escherichia coli K-12 substr. MG1655)
ABC-19-RXN [EC: 7.3.2.5]; ABC-7-RXN [EC: 7.3.2.5, 7.3.2.3]; 7.3.2.3 [EC: 7.3.2.5, 7.3.2.3]; TRANS-RXN0-478 [EC: 7.3.2.5, 7.3.2.3]; TRANS-RXN0-479 [EC: 7.3.2.5, 7.3.2.3]

Predicted SEED Role

"Sulfate and thiosulfate binding protein CysP" in subsystem Cysteine Biosynthesis

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.3.2.3 or 7.3.2.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RNV1 at UniProt or InterPro

Protein Sequence (354 amino acids)

>Rru_A3400 Thiosulphate-binding protein (NCBI) (Rhodospirillum rubrum S1H)
MTRQLVSRSRVSGRRLIPLIVAGLLASAPVPGLIAQAEAKDVTLLNVSYDPTRELYKAVN
AAFAESWKAKTGDTVIINTSHGGSGKQARAVIDGLEADVVTLALASDIDAIATHTKRLSP
DWQKRLPDASSPYTSTIVFLVRKGNPKGIHDWDDLVREGVQVITPNPKTSGGARWNHLAA
WGYAEQKFGGDQAKVKDFIKTLYKNVPVLDTGARGSTTTFVKRGLGDVLLAWENEAFLAI
EELGPNQFDIVVPSLSILAQPPVALVDGEVDRHGTREVAQAYLEFLYTPAAQKIIAHNFY
RPVNREAADPADIARFPEVKLLTIDDPVFGGWTKAQKEHFDEGGLFDQIYQPGG