Protein Info for Rru_A3366 in Rhodospirillum rubrum S1H

Annotation: Cobaltochelatase, CobN subunit (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

KEGG orthology group: K02230, cobaltochelatase CobN [EC: 6.6.1.2] (inferred from 54% identity to apb:SAR116_0571)

Predicted SEED Role

"CobN component of cobalt chelatase involved in B12 biosynthesis" in subsystem Coenzyme B12 biosynthesis

MetaCyc Pathways

adenosylcobalamin biosynthesis II (aerobic) (29/33 steps found)
cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation) (12/13 steps found)

KEGG Metabolic Maps

Porphyrin and chlorophyll metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.6.1.2

Use Curated BLAST to search for 6.6.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RNY5 at UniProt or InterPro

Protein Sequence (1240 amino acids)

>Rru_A3366 Cobaltochelatase, CobN subunit (NCBI) (Rhodospirillum rubrum S1H)
MHLLAAQPGRVSDGSDAVDLGQDPADILFLSAADTEIACLAAARAAWAEAPSLRAANLTR
LGHPMSVDLWIDDLASRAKLVVVRLLGGRGYWPHGVDSLADLARTRGLTLALLPGDANPD
VELARLSTLPRETLHRLWRYWVEGGIDNAGQFLRCCAELIGRPTPWRDPVPLPVAGLLGD
EPDAADGRSGVALVFYRALVQAGDLAPITALRAALEARGLRVVALYCTSLKDPLAAATVE
TVLLRQSPAVIINFTGFAVSAPGAPRVSPLAGPDAPVLQAILAASDEIGWRQLANGLIAR
DIAMNVALPEVDGRILSRAISFKALDERDEALQTTLTRHRPVADRVDFVAALAANWARLR
ATAPGERRVALILANYPNRDGRVGNGVGLDTPAATVRVLRALEAAGYGVEGAPGDGAGLM
ERLLRQGPTNALGSAGRSGGGVLLPLAAYQAFLAALPGQAGHKVTERWGRPEEDPFYVAG
RGGFVLPLAVMGRVVVGIQPARGYNLDPVASYHDPALPPPHGYLAFHAWLRASFDVHAIV
HMGKHGNLEWLPGKAVALSQDCFPEAALGPLPHLYPFIVNDPGEGTQAKRRSQAVIIDHL
TPPLTRAETYGVLRDLERLVDEFYEAASIDPRRLGVLKRAILDLAENAGLLADLGLGKGI
NDEDALSALDNHLCDLKEMQIRDGLHVFGDSPVGRLRTDLLVALARGPRGAGAANESLTR
ALARDLGLGAFDPLAATLAEPWQGPRPDALADQVEDPWRTAGDTVERLEYLASALVSGAR
PADPAWPRTAAVLDTVFTTLGPAVDACGPAEIAGLLSGLDGRFVDPGPSGAPSRGRPDVL
PTGRNFYSVDNRTVPTPAAWTLGWKAANLLLERHLQDHGDWPRSLVLTAWGTSTMRTGGD
DIAQTLALMGVQTTWDPMTRRVTGFEIMPLSVLDRPRVDVTLRISGFFRDAFPGLVDLVD
SAARAVAAQDDEPADLNPLAQRVREETQQMVRSGIEPALARRRAGHRVFGARPGAYGAGL
QALIDERGWETDADLARAYIAWGGYAYGDGAAGDAAHGQFQRRLAAAEAVVQNQDNREHD
LLDSDDYYQFEGGAAAAVRVLSGNQPAIYHGDTSRTDSPRIRTLTEEIARVVRARVVNPK
WIRGAMRHGYKGAFEMAATVDYLFAFAATARVVKDHQFDLVAEAYLLDPEVRGFLRAVNP
DALAEMARRLREALDRGLWRSRRNDMGAVLAALAEGQEPS