Protein Info for Rru_A3352 in Rhodospirillum rubrum S1H

Annotation: Pseudouridylate synthase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 278 TIGR00071: tRNA pseudouridine(38-40) synthase" amino acids 6 to 239 (234 residues), 185 bits, see alignment E=8.6e-59 PF01416: PseudoU_synth_1" amino acids 8 to 105 (98 residues), 46.3 bits, see alignment E=2.7e-16 amino acids 144 to 246 (103 residues), 102.8 bits, see alignment E=7.1e-34

Best Hits

Swiss-Prot: 100% identical to TRUA_RHORT: tRNA pseudouridine synthase A (truA) from Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIB 8255 / S1)

KEGG orthology group: K06173, tRNA pseudouridine synthase A [EC: 5.4.99.12] (inferred from 100% identity to rru:Rru_A3352)

Predicted SEED Role

"tRNA pseudouridine synthase A (EC 4.2.1.70)" in subsystem tRNA processing (EC 4.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RNZ9 at UniProt or InterPro

Protein Sequence (278 amino acids)

>Rru_A3352 Pseudouridylate synthase (NCBI) (Rhodospirillum rubrum S1H)
MVRYRLTVEYDGRGYCGWQRQDNGPTIQQSLEEAAFRLCGVATRVHGSGRTDSGVHALGQ
VAHLDLPRAYGAATVMKALNAHLRPQPIAVIDAAEVAEDFHARFSAEERSYRYRILNRIA
PPTLDQGRVWWVARPMEAAIMDEAAQVLVGRHDFSSFRAAECQADSPVKTLSELRVTRVG
DEIHVFARARSFLHHQVRNMVGTLALVGDGRWTADRLRAALEACDRSAAGPTAPPDGLYF
LRVRFPGETAAPAKAGPLEAAPLGEAPLKEATLKEDWR