Protein Info for Rru_A3214 in Rhodospirillum rubrum S1H
Annotation: O-succinylhomoserine sulfhydrylase (NCBI)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 53% identical to METZ_MYCTU: O-succinylhomoserine sulfhydrylase (metZ) from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
KEGG orthology group: K10764, O-succinylhomoserine sulfhydrylase [EC: 2.5.1.-] (inferred from 100% identity to rru:Rru_A3214)Predicted SEED Role
"O-acetylhomoserine sulfhydrylase (EC 2.5.1.49)" in subsystem Methionine Biosynthesis (EC 2.5.1.49)
MetaCyc Pathways
- superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
- superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (9/10 steps found)
- L-methionine biosynthesis III (4/4 steps found)
- superpathway of L-cysteine biosynthesis (fungi) (5/6 steps found)
- L-homocysteine biosynthesis (2/2 steps found)
- S-methyl-5-thio-α-D-ribose 1-phosphate degradation II (4/5 steps found)
- S-methyl-5-thio-α-D-ribose 1-phosphate degradation III (4/5 steps found)
KEGG Metabolic Maps
- Biosynthesis of terpenoids and steroids
- Carotenoid biosynthesis - General
- Cysteine metabolism
- Methionine metabolism
- Porphyrin and chlorophyll metabolism
- Riboflavin metabolism
- Terpenoid biosynthesis
- Tryptophan metabolism
- Ubiquinone and menaquinone biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 2.5.1.-, 2.5.1.49
Use Curated BLAST to search for 2.5.1.- or 2.5.1.49
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q2RPD6 at UniProt or InterPro
Protein Sequence (413 amino acids)
>Rru_A3214 O-succinylhomoserine sulfhydrylase (NCBI) (Rhodospirillum rubrum S1H) MPRESDFSPAQSPSLEAPTGPATWRADTLLVRGGLARTGLNETSEALFLNSGYVYPNAEE AEAAFDGTLERYVYSRFRNPTISVFEERLAALEGAPVCKATASGMAAVTSALLCQVRAGD RVVAARDLFGSCSWVIGDLLAQYGVSAEFVDTENLDAWAQALAKPTRAVFLETPSNPTLR IVDLKGVCDLAHAAGATVVVDNAFATPLLQRPRDFGADVVVHSATKWIDGQGRCLGGAVL CDEAFNETYLGPFLRHTGPCLAPFNAWVMLKGLETLSLRINRHSATALTLAGLIEGHPAV ARALYPGLASHPRHALAQSQMKAGGGVIALSLKGGRASAYRFLNALSIVDISNNLGDAKS LACHPWTTTHQRLSPEEKLIQGIDEGLIRFSVGLEDPEDLAADIGAALDAAGG