Protein Info for Rru_A3000 in Rhodospirillum rubrum S1H

Annotation: Formate acetyltransferase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01255: formate acetyltransferase
  • amino acids 14 to 752 (739 residues), 1163.8 bits, E=0
• PF02901: PFL-like
  • amino acids 26 to 611 (586 residues), 707.8 bits, E=1.6e-216
• PF01228: Gly_radical
  • amino acids 627 to 733 (107 residues), 114 bits, E=4.8e-37

Best Hits

Swiss-Prot: 66% identical to PFLB_STAA3: Formate acetyltransferase (pflB) from Staphylococcus aureus (strain USA300)

KEGG orthology group: K00656, formate C-acetyltransferase [EC: 2.3.1.54] (inferred from 100% identity to rru:Rru_A3000)

MetaCyc: 64% identical to pyruvate formate-lyase (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Pyruvate formate-lyase (EC 2.3.1.54)" in subsystem Butanol Biosynthesis or Fermentations: Mixed acid (EC 2.3.1.54)

MetaCyc Pathways

• L-threonine degradation I (6/6 steps found)
• pyruvate fermentation to acetate IV (3/3 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (10/13 steps found)
• reductive monocarboxylic acid cycle (2/2 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (14/19 steps found)
• superpathway of L-threonine metabolism (13/18 steps found)
• pyruvate fermentation to ethanol I (2/3 steps found)
• superpathway of fermentation (Chlamydomonas reinhardtii) (6/9 steps found)
• mixed acid fermentation (11/16 steps found)
• superpathway of N-acetylneuraminate degradation (13/22 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Propanoate metabolism
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.54

Use Curated BLAST to search for 2.3.1.54

Sequence Analysis Tools

See Q2RQ00 at UniProt or InterPro

Protein Sequence (752 amino acids)

>Rru_A3000 Formate acetyltransferase (NCBI) (Rhodospirillum rubrum S1H)
MSIVQKDIPATNPWKGFTEGPWQTGIDVRDFIQKNVTPYQGDGAFLAGPTDRTRLLWEKL
SALLLKEREKGVLDVSADRASSITAHDAGYIDRDAEIIVGLQTDAPLKRAIMPNGGLRMV
QSGLEAYGFASDPTVNEVWTKYRKSHNQGIFDVYSPDVLAARKSGVITGLPDAYGRGRII
GDYRRVALYGVDFLRDQKQMEFHELDDAVFSEDVIRLREELSEQWRALDELKQMAAKYGH
GIGRPAANAREAIQWTYFAYLAAVKEQNGAAMSLGRVSSFLDIYIQRDIEAGLLSEDGAQ
EMIDDLVIKLRIVRFLRTPDYDQLFSGDPTWVTECVGGMAEDGRTLVTRNSFRFLNTLYN
LGPAPEPNLTVLWSTALPQSFKTFCAKVSADTSAIQYENDDLMRRRWGDDYGIACCVSAM
RIGKQMQFFGARANLAKALLYAINGGVDEKSGQVVAKGFAPITADVLDYDEVMAKLDKTM
DWLAQTYVKALNTIHYMHDKYAYERIEMALHDRDILRTMACGIAGLSVAADSLSAIKFAK
VHVVRDDKGLAVDYTIEGDYPAYGNNDDRADSIAVWLTETFMEKVRAQPRFYRDAMPTQS
VLTITSNVVYGKKTGNTPDGRRAGEPFAPGANPMNGRDVKGFVASGASVAKLPYAAALDG
ISWTASATPDSLGRTDEERAVNLSNCLDAFATAGGFHVNVNVFNRDTLLHAMDHPELYPQ
LTIRVSGYAVNFVKLTREQQLDVIGRTFHQTM