Protein Info for Rru_A2818 in Rhodospirillum rubrum S1H

Annotation: Alanine--tRNA ligase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01411: tRNA-synt_2c
  • amino acids 6 to 557 (552 residues), 693.5 bits, E=3.2e-212
• TIGR00344: alanine--tRNA ligase
  • amino acids 6 to 864 (859 residues), 936.2 bits, E=1e-285
• PF07973: tRNA_SAD
  • amino acids 654 to 705 (52 residues), 55.6 bits, 6.2e-19
• PF02272: DHHA1
  • amino acids 742 to 881 (140 residues), 72.9 bits, E=5.8e-24

Best Hits

Swiss-Prot: 70% identical to SYA_MAGSA: Alanine--tRNA ligase (alaS) from Magnetospirillum magneticum (strain AMB-1 / ATCC 700264)

KEGG orthology group: K01872, alanyl-tRNA synthetase [EC: 6.1.1.7] (inferred from 69% identity to azl:AZL_a04560)

Predicted SEED Role

"Alanyl-tRNA synthetase (EC 6.1.1.7)" (EC 6.1.1.7)

MetaCyc Pathways

• tRNA charging (19/21 steps found)
• shinorine biosynthesis (3/8 steps found)
• vanchrobactin biosynthesis (2/8 steps found)
• ergotamine biosynthesis (4/12 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
• superpathway of chorismate metabolism (37/59 steps found)
• bacillibactin biosynthesis (3/12 steps found)
• enterobactin biosynthesis (2/11 steps found)
• superpathway of ergotamine biosynthesis (5/18 steps found)
• sulfazecin biosynthesis (1/16 steps found)
• phosalacine biosynthesis (4/25 steps found)
• phosphinothricin tripeptide biosynthesis (4/25 steps found)
• corallopyronin A biosynthesis (2/30 steps found)
• colibactin biosynthesis (6/38 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Aminoacyl-tRNA biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.7

Sequence Analysis Tools

See Q2RQI0 at UniProt or InterPro

Protein Sequence (883 amino acids)

>Rru_A2818 Alanine--tRNA ligase (NCBI) (Rhodospirillum rubrum S1H)
MSTTSEIRRTFLDFFVKNGHQEVASSPLVPLNDPTLMFTNAGMVQFKNVFTGAETRPYHR
ATTSQKCVRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYFKEQAIDFAWRLITEEYAL
PKDRLLVTVHTSDEDAAGLWRKVAGLPDERIIRIPTNDNFWAMGDTGPCGPCSEIFFDHG
PKVAGGPPGSADENGDRFIEIWNLVFMQFEQLTVDQRVALPRPSIDTGMGLERVAAVLQG
KHDNYDIDLMRALIEAIASAAGTDPDGPHKVSHRVIADHLRSACFLIADGVLPSNEGRGY
VLRRIMRRAMRHAHMMGCVDPLMFKLVPALNREMADQFPELNRANALITETLKLEETRFK
QMLDRGLKLLAEETGDLASGDSLPGPVAFKLYDTYGFPLDLTQDALRPRGIGVDTAGFDA
AMARQREDARKSWTGSGDHATEAVWFDIRDRVGASEFLGYTSTDAEGAIVALVVDGKVVE
SAPADTEVAVIANQTPFYGESGGQMGDAGEIRLAEGGKVVVRDTAKKLGALHVHIGRVEG
ATIRVGQAARFVVDTERRDALRSHHSATHLLHEALRRRLGEHVTQKGSLVAPDRLRFDIS
HPKPLSAAEIRVVEDDVNREIRANAEITTQIMDPDSAIEAGAMALFGEKYGEEVRVVSMG
RREPGANRPFSVELCGGTHARRTGDIGFFKIIGEGAVASGVRRIEAVTGQAAFEHIEAQD
DLVSGAAQLLKVTPADLPGRIQALLDDRRRLERDLADARRKLATGGGGAAAPAVVEINGV
KWVGRLLEDVPAKDLKGMVDEVKKQLGSGVVALIAVADGKASLVVGVTEDLVGRFDAVAL
VRAGAGAIGGKGGGGRPDMAQAGGPDGAQAGAALAAIEAALAG