Protein Info for Rru_A2630 in Rhodospirillum rubrum S1H
Annotation: ROK (NCBI)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 55% identical to MAK_ECOLI: Fructokinase (mak) from Escherichia coli (strain K12)
KEGG orthology group: None (inferred from 100% identity to rru:Rru_A2630)MetaCyc: 55% identical to fructokinase (Escherichia coli K-12 substr. MG1655)
Mannokinase. [EC: 2.7.1.7]; Fructokinase. [EC: 2.7.1.7, 2.7.1.4]
Predicted SEED Role
"ROK family Glucokinase with ambiguous substrate specificity"
MetaCyc Pathways
- superpathway of anaerobic sucrose degradation (15/19 steps found)
- D-mannose degradation II (2/2 steps found)
- sucrose degradation II (sucrose synthase) (4/5 steps found)
- mannitol degradation II (3/4 steps found)
- sucrose degradation III (sucrose invertase) (3/4 steps found)
- sucrose degradation IV (sucrose phosphorylase) (3/4 steps found)
- D-sorbitol degradation I (2/3 steps found)
- 1,5-anhydrofructose degradation (3/5 steps found)
- sucrose degradation I (sucrose phosphotransferase) (1/3 steps found)
- mannitol cycle (2/5 steps found)
- CMP-2-keto-3-deoxy-D-glycero-D-galacto-nononate biosynthesis (1/4 steps found)
- sucrose degradation VII (sucrose 3-dehydrogenase) (1/4 steps found)
- heterolactic fermentation (11/18 steps found)
- superpathway of CMP-sialic acids biosynthesis (2/15 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.7.1.4
Use Curated BLAST to search for 2.7.1.4 or 2.7.1.7
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q2RR18 at UniProt or InterPro
Protein Sequence (310 amino acids)
>Rru_A2630 ROK (NCBI) (Rhodospirillum rubrum S1H) MTLRLGLDLGGTKTEIIALDDEGRILLRRRRPSPRAAYGATLDCLAALVTEAEAELGRQG SVGVAMPGAISPASGLVKNANSHWLNGQRLDHDLAERLGRPVRVANDADCFALSEATDGA AAGASSVFGVILGTGVGAGIVVNGRLLAGPNAIAGEWGHMPLPWPGDDERPGPDCYCGLK GCVETFCSGPGLAADHQKSTGHAIEGPALLALAQAGDAQAQASLDRHADRLARALAVVIN ILDPQVIVLGGGLGQMPHLYQALPRLWTPWVFSDRVDTRLVAPRHGDSSGVRGAAWLWPP SSTLETDPHA