Protein Info for Rru_A2576 in Rhodospirillum rubrum S1H

Annotation: 1,4-alpha-glucan branching enzyme (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 740 TIGR01515: 1,4-alpha-glucan branching enzyme" amino acids 114 to 731 (618 residues), 986.3 bits, see alignment E=3e-301 PF02922: CBM_48" amino acids 129 to 213 (85 residues), 77.7 bits, see alignment E=1.1e-25 PF00128: Alpha-amylase" amino acids 282 to 354 (73 residues), 33.5 bits, see alignment E=5.4e-12 PF02806: Alpha-amylase_C" amino acids 635 to 732 (98 residues), 94 bits, see alignment E=8.7e-31

Best Hits

Swiss-Prot: 100% identical to GLGB_RHORT: 1,4-alpha-glucan branching enzyme GlgB (glgB) from Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIB 8255 / S1)

KEGG orthology group: K00700, 1,4-alpha-glucan branching enzyme [EC: 2.4.1.18] (inferred from 100% identity to rru:Rru_A2576)

MetaCyc: 54% identical to 1,4-alpha-glucan branching enzyme (Mycobacterium tuberculosis H37Rv)
1,4-alpha-glucan branching enzyme. [EC: 2.4.1.18]

Predicted SEED Role

"1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 2.4.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.18

Use Curated BLAST to search for 2.4.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RR72 at UniProt or InterPro

Protein Sequence (740 amino acids)

>Rru_A2576 1,4-alpha-glucan branching enzyme (NCBI) (Rhodospirillum rubrum S1H)
MDPKAYSEAVAALVGARHSNPFAFLGPHEGRAEGGRGGFCIRCFRPKATAVSLISAADDT
VLGRMKRLHPDGLFGIDLPEAPGALAYRLRVSEGDEDRDIEDPYRFGPVLGELDRHLLGE
GTHLDIYRKMGAHPMTRDGVRGTGFALWAPNATRVSVIGDFNGWDGRLHPMRAHPGSGVW
DIFLPGVVEGHLYKYELLGPDGSLLPAKADPYAFQAEKPPHTASVVRGLGTYAWNDGRWM
SERADRVATSAPVSIYEVHLGSWRHGGGDRSLSYREMAEQLIPYVKEMGFTHIELLPVSE
FPFDGSWGYQPIGLFAPTSRFGEPDDFRHFVDRCHQEGVGVILDWVAGHFPEDAHGLSWF
DGTHLYEHSDPRQGRHMDWGTYIFNYGRNEVRNFLLANALFWLEQFHIDGLRVDAVASML
YLDYSRKAGEWVPNKFGGRENLEAIDFLRRMNELVYGRFPGAVTIAEESTAWPMVSRPVH
LGGLGFGYKWNMGWMNDTLSYMSQDPIYRRFHQHDLSFGLLYAFTENFVLPLSHDEVVHG
KRSILGRMPGDAWQQFANLRAYYGFMWTHPGKKLLFMGCEFAQGREWNHNASLDWHLLDI
DWHKGVQALVRDLNGLYAGVPALHDRDTEGYGFSWIDCTDADQSVLAFLRFGETAEDVVM
VVCNFTPNPRHGYRLGAPIAGRWREIFNTDSAHYGGSNMGNSVVETEETKSHGHAQSVVL
TLPPLATIVLRPDGPITRIA