Protein Info for Rru_A2479 in Rhodospirillum rubrum S1H

Annotation: Methylmalonyl-CoA mutase-like (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 732 transmembrane" amino acids 166 to 184 (19 residues), see Phobius details TIGR00641: methylmalonyl-CoA mutase N-terminal domain" amino acids 41 to 562 (522 residues), 908.6 bits, see alignment E=9.3e-278 PF01642: MM_CoA_mutase" amino acids 44 to 555 (512 residues), 764.5 bits, see alignment E=5.1e-234 TIGR00640: methylmalonyl-CoA mutase C-terminal domain" amino acids 595 to 723 (129 residues), 205.8 bits, see alignment E=2.1e-65 PF02310: B12-binding" amino acids 598 to 711 (114 residues), 65.8 bits, see alignment E=3.4e-22

Best Hits

Swiss-Prot: 76% identical to MUTB_STRCM: Methylmalonyl-CoA mutase large subunit (mutB) from Streptomyces cinnamonensis

KEGG orthology group: K01847, methylmalonyl-CoA mutase [EC: 5.4.99.2] (inferred from 100% identity to rru:Rru_A2479)

MetaCyc: 72% identical to methylmalonyl-CoA mutase large subunit (Chloroflexus aurantiacus)
Methylmalonyl-CoA mutase. [EC: 5.4.99.2]

Predicted SEED Role

"Methylmalonyl-CoA mutase (EC 5.4.99.2)" in subsystem Propionyl-CoA to Succinyl-CoA Module or Serine-glyoxylate cycle (EC 5.4.99.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 5.4.99.2

Use Curated BLAST to search for 5.4.99.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RRG6 at UniProt or InterPro

Protein Sequence (732 amino acids)

>Rru_A2479 Methylmalonyl-CoA mutase-like (NCBI) (Rhodospirillum rubrum S1H)
MSRIPDFSRVDLEGGEAPGSADLWTDRIEHRAGKAVRDLLWTAPEKIDVKPLYQASDLDG
LDFLDTYPGAPPFLRGPYPSMYVNQPWTVRQYAGFSTATESNAFYRRNLAAGQKGLSVAF
DLATHRGYDSDHPRVAGDVGMAGVAIDSIFDMRTLFAGIPLDKMSVSMTMNGAVLPIMAL
FILAGEEQGVSPEQLSGTIQNDILKEFMVRNTYIYPPTPSMRIISDIFAYTSTKMPRFNS
ISISGYHMQEAGATADLELAYTLADGVEYARAGLAAGLTIDAFAPRLSFFWAIGMNFPME
VAKMRAARLLWAKLIKDFKPKSDKSLSLRTHCQTSGWSLTAQDVFNNVTRTCVEAMAATQ
GHTQSLHTNALDEALALPTDFSARIARNTQLFLQQEAGTCRVIDPWGGSYYIERLTHDLA
ARAWHYIQEVEETGGMAKAIEAGIPKMRIEEASARTQARIDSGRQTVVGVNKYKVDEDRS
IEVLKVENASVRRQQIEQLERLRAERDPAKVEEKLAALTHSAQSGEGNLLALAVDAVREK
ATVGEVSLALEKVWGRHKAEIRAISGVYSSEVGSMAEKVASIRHLVEEFEANDGRRPRIL
IAKMGQDGHDRGQKVIATAFADLGFDVDIGSLFQTPAETARQAVENDVHVVGASSLAAGH
LTLVPQLREELRKQGREDIMIVVGGVIPPQDFDALYKAGAEAIFPPGTNIAECAVDLMGK
LNARLGYTSKAA