Protein Info for Rru_A2295 in Rhodospirillum rubrum S1H

Annotation: malto-oligosyltrehalose trehalohydrolase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 672 PF02922: CBM_48" amino acids 51 to 112 (62 residues), 26 bits, see alignment E=1.5e-09 TIGR02402: malto-oligosyltrehalose trehalohydrolase" amino acids 60 to 603 (544 residues), 609.9 bits, see alignment E=1.8e-187 PF00128: Alpha-amylase" amino acids 169 to 269 (101 residues), 42.5 bits, see alignment E=8.4e-15 PF11941: DUF3459" amino acids 549 to 638 (90 residues), 54.7 bits, see alignment E=1.7e-18

Best Hits

KEGG orthology group: K01236, maltooligosyltrehalose trehalohydrolase [EC: 3.2.1.141] (inferred from 100% identity to rru:Rru_A2295)

Predicted SEED Role

"Malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141)" in subsystem Trehalose Biosynthesis (EC 3.2.1.141)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.141

Use Curated BLAST to search for 3.2.1.141

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RS00 at UniProt or InterPro

Protein Sequence (672 amino acids)

>Rru_A2295 malto-oligosyltrehalose trehalohydrolase (NCBI) (Rhodospirillum rubrum S1H)
MESPLTPPVAPAPRPPLSSLVAAPPAEDPPSARSVAAAPPPAPPVRRLPQGAEVQPGGGV
HFRVWAPGRQAVTLCLENGAAIAMTAEDDGSFAALVAEAGAGTAYRYRLEGVEALCPDPL
ARFQPEGPHGPSLVVDPAGFVWSDQAWAGVSHQGMVLYEMHIGTFTPEGTWAAAMAHLER
LRDLGVTCLEIMPIAEFSGRFGWGYDGVNLFAPTRLYGQPDDMRRFVDRAHALGLAVILD
VVYNHLGPDGNYLGHFTNDYFTDRYKNDWGVAINFDGPGSAGVRAFYLANAGYWIEEFHL
DGLRLDATQQIYDRSEPSILAEIAREVRAAAKGRNTFVVAENEPQEVRLIEDETAQGYGL
DALWNDDLHHAAMVALTGRREAYYLDYLGSPQELISAAKWGFLYAGQYYSWQRNGRGTTS
FAIPGRAMVAFLQNHDQVANSARGLRLDKLSDEAQFRALTAYLLLIPATPMLFQGQEFRS
SAPFYYFADHGPKLAAMVKDGRADFLTQFPSIDQGWRSEDLPDPAAEATFERCKLDHREW
QSHAEWVALHRDLLTLRRETPAFAAQDCRRMEGAVLGPQTFVLRFFAEERLSDRLMLVNL
GPQITLTPMAEPLLAPPMGKAWTTLWASSDQRYGGAGLTAVERTPLWEIPARTTVVLGPG
EKKPFIAPRTSG