Protein Info for Rru_A2290 in Rhodospirillum rubrum S1H

Annotation: Sulfate adenylyltransferase, large subunit (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00009: GTP_EFTU
  • amino acids 24 to 210 (187 residues), 140.9 bits, E=9.2e-45
• TIGR02034: sulfate adenylyltransferase, large subunit
  • amino acids 24 to 430 (407 residues), 637.8 bits, E=1.2e-195
• TIGR00231: small GTP-binding protein domain
  • amino acids 29 to 209 (181 residues), 44.2 bits, E=2.7e-15
• PF22594: GTP-eEF1A_C
  • amino acids 332 to 431 (100 residues), 94.1 bits, E=1.6e-30
• TIGR00455: adenylyl-sulfate kinase
  • amino acids 444 to 623 (180 residues), 238.6 bits, E=5.7e-75
• PF01583: APS_kinase
  • amino acids 459 to 609 (151 residues), 222.1 bits, E=8.6e-70

Best Hits

Swiss-Prot: 59% identical to NODQ_RHIS3: Bifunctional enzyme NodQ (nodQ) from Rhizobium sp. (strain N33)

KEGG orthology group: K00955, bifunctional enzyme CysN/CysC [EC: 2.7.1.25 2.7.7.4] (inferred from 100% identity to rru:Rru_A2290)

Predicted SEED Role

"Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) / Adenylylsulfate kinase (EC 2.7.1.25)" in subsystem Cysteine Biosynthesis or O-Methyl Phosphoramidate Capsule Modification in Campylobacter (EC 2.7.1.25, EC 2.7.7.4)

MetaCyc Pathways

• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (9/10 steps found)
• assimilatory sulfate reduction I (4/4 steps found)
• assimilatory sulfate reduction IV (4/4 steps found)
• assimilatory sulfate reduction III (3/3 steps found)
• sulfate activation for sulfonation (2/2 steps found)
• assimilatory sulfate reduction II (2/3 steps found)
• sulfite oxidation III (1/3 steps found)
• selenate reduction (2/5 steps found)
• dissimilatory sulfate reduction I (to hydrogen sufide)) (1/5 steps found)
• superpathway of sulfur metabolism (Desulfocapsa sulfoexigens) (1/6 steps found)
• superpathway of thiosulfate metabolism (Desulfovibrio sulfodismutans) (1/6 steps found)
• superpathway of sulfide oxidation (phototrophic sulfur bacteria) (2/12 steps found)
• superpathway of seleno-compound metabolism (6/19 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.4

Use Curated BLAST to search for 2.7.1.25 or 2.7.7.4

Sequence Analysis Tools

See Q2RS05 at UniProt or InterPro

Protein Sequence (641 amino acids)

>Rru_A2290 Sulfate adenylyltransferase, large subunit (NCBI) (Rhodospirillum rubrum S1H)
MDGVSDAARQVENYLAHHEDKGLLRLLTCGSVDDGKSTLIGRLLHDCKLIFDDLMGALER
DSKTSGTTGPGKLDFALLLDGLQAEREQGITIDIAYRYFATDKRKFIVADAPGHEQYTRN
MATAASTAELAILLIDARKGVITQTRRHTAVCAMMGVRKLVLAVNKMDLVGYEQATFDRI
VADYRAFAAKLGIEAVTAIPLSALEGQNMIVRSPAMPWYTGPALLPYLEEVDVTTDRELL
PMRFPVQWVNRPNLDFRGFSGTLVSGAVSVGDGVSVLPGGQTSRVKRIVTKDGDLPRAVA
GQAITLTLADEIDISRGDLLVPTGDQPAQADHFAAHLLWMDAKALMPGRDYLVRMGPLQM
RGQITAIKHKIDVNTMEERPAPTLELNDVGSVNLQVDRAVPFDPYDQNRDTGSFILIDRL
TNATVGAGMIRFSLWRSTNLVWQHIEVGAAQRAAQKGQKPCVIWLTGLSGSGKSTIANLL
DKRLYAEAHHTTMLDGDNVRHGLNRDLGFTEQDRVENIRRVAEVAKLMADAGLITLVSFI
SPYREERLAARARIGEERFVEVYVKASVADCAARDPKGLYRKAMAGEIRNFTGIDAPYEE
PVTPDLLIDTAVLTPEAAVEVLMAWLRAKGYLSAESEGSGI