Protein Info for Rru_A2276 in Rhodospirillum rubrum S1H

Annotation: Binding-protein-dependent transport systems inner membrane component (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 314 transmembrane" amino acids 9 to 29 (21 residues), see Phobius details amino acids 100 to 122 (23 residues), see Phobius details amino acids 136 to 160 (25 residues), see Phobius details amino acids 176 to 195 (20 residues), see Phobius details amino acids 230 to 255 (26 residues), see Phobius details amino acids 276 to 301 (26 residues), see Phobius details TIGR02789: nickel ABC transporter, permease subunit NikB" amino acids 1 to 308 (308 residues), 487.5 bits, see alignment E=9.7e-151 PF19300: BPD_transp_1_N" amino acids 1 to 104 (104 residues), 37.9 bits, see alignment E=1.8e-13 PF00528: BPD_transp_1" amino acids 117 to 307 (191 residues), 151.1 bits, see alignment E=3e-48

Best Hits

Swiss-Prot: 71% identical to NIKB_ECOLI: Nickel transport system permease protein NikB (nikB) from Escherichia coli (strain K12)

KEGG orthology group: K02033, peptide/nickel transport system permease protein (inferred from 100% identity to rru:Rru_A2276)

MetaCyc: 71% identical to nickel ABC transporter membrane subunit NikB (Escherichia coli K-12 substr. MG1655)
7.2.2.i [EC: 7.2.2.i]; 7.2.2.- [EC: 7.2.2.i]

Predicted SEED Role

"Nickel transport system permease protein NikB (TC 3.A.1.5.3)" in subsystem Transport of Nickel and Cobalt (TC 3.A.1.5.3)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.2.2.i

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RS19 at UniProt or InterPro

Protein Sequence (314 amino acids)

>Rru_A2276 Binding-protein-dependent transport systems inner membrane component (NCBI) (Rhodospirillum rubrum S1H)
MVSFVLKRLALLVPMLLGASLVIFLMLRLGPSDPAMDYLRLSRIPPTVQALAETRQRLGL
DQPLAVQYWVWLNKALHLDFGVSYATGRPVLPDLLHYLPATLHLAGVALALTLGLSIPLG
MWAARRRDALPDQIVRGVAFLGVSMPNFWLGFLLVLVFSVELGWLPAMGRGGAEHMIMPA
VAIAFMSLSINARLLRASMLEVSGQRHVYYARLRGLSAAKVERAHILRNALLPIITATGM
HVGELIGGTLVIESIFGWPGVGRFAVSAIFNRDYPVIQCFTLLMVLIFVLCNLIVDIVYA
WADPRIRLAAEGAQ