Protein Info for Rru_A2244 in Rhodospirillum rubrum S1H

Annotation: Glycogen/starch/alpha-glucan phosphorylase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 826 TIGR02093: glycogen/starch/alpha-glucan phosphorylases" amino acids 20 to 819 (800 residues), 1156.3 bits, see alignment E=0 PF00343: Phosphorylase" amino acids 108 to 819 (712 residues), 1090.8 bits, see alignment E=0

Best Hits

Swiss-Prot: 53% identical to PYGB_BOVIN: Glycogen phosphorylase, brain form (PYGB) from Bos taurus

KEGG orthology group: K00688, starch phosphorylase [EC: 2.4.1.1] (inferred from 100% identity to rru:Rru_A2244)

MetaCyc: 52% identical to glycogen phosphorylase b (Homo sapiens)
Phosphorylase. [EC: 2.4.1.1]; 2.4.1.1 [EC: 2.4.1.1]; 2.4.1.1 [EC: 2.4.1.1]

Predicted SEED Role

"Glycogen phosphorylase (EC 2.4.1.1)" in subsystem Glycogen metabolism or Maltose and Maltodextrin Utilization (EC 2.4.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RS51 at UniProt or InterPro

Protein Sequence (826 amino acids)

>Rru_A2244 Glycogen/starch/alpha-glucan phosphorylase (NCBI) (Rhodospirillum rubrum S1H)
MDTVIRTDFELDEGIDFTDVEQIKHGLVTSMVRGVGFDPASATEHDWFLALASLLRGHLS
EKGMMTSRAQYGSDTKRVYYLSLEFLTGRRLVKHLLDLGIESAVRAALRELGQDLDRVAE
QESDAALGNGGLGRLAACFLDSMATHGYPGYGYGIRYEFGMFSQTIENGQQVEHPESWLR
NGNPWEIVRHNVSYPVRFGGHIVCFRDEGGEERCRWVDANEVIAEAYDLKETGYGGAYGC
NLRLWSARATQDFDLSYFNEGNYIEAVKDKTTSENLSKVLYPMDTTLMGQELRLKQEYFF
VSASLQDILARFFKVHKDPKQIPAKIVIQLNDTHPALAVPEMMRLLMDNHALSWDAAWDI
TRDTFAYTNHTLLPEALETWPVAMLEAILPRHLEIIYKINYGFLQQVRRAFPGDVAMLRR
MSLIDEDTHRVRMAHLAVVGSRRVNGVAALHTKLLRERVFPDFDAFFPEKFVNVTNGITQ
RRWLLQSNPPLAALVSETIGEGWLTDLDRLRDLEKLADDPAFQDRFLTIKAGAKARAAAL
IAERCGVVVSTASLFDIQIKRIHEYKRQLLNIMQVIARYNAIRRDPKAVMTPRTVIFGGK
AAPGYYVAKKIIRLINDVAETINHDPAVRDLLKVVFVPNYNVSTAEILIPSCDLSEQIST
AGTEASGTGNMKFALNGALTIGTLDGANIEIREEVGDDNIFIFGHTAQEVSQIKAQGYDP
WSWYAADDEMRTVIDMIRDGFFSTEEPDRYRPIADALLGSDTYLVLADFRSYMDAQKAVD
GLYTNQRVWARQAILNIARVGKFSSDRAIHTYAKDIWGVRPMDALS