Protein Info for Rru_A2148 in Rhodospirillum rubrum S1H

Annotation: N-acetylmuramoyl-L-alanine amidase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 522 signal peptide" amino acids 1 to 46 (46 residues), see Phobius details PF11741: AMIN" amino acids 57 to 128 (72 residues), 29.2 bits, see alignment E=8.4e-11 PF01520: Amidase_3" amino acids 295 to 508 (214 residues), 162.5 bits, see alignment E=1.1e-51

Best Hits

KEGG orthology group: K01448, N-acetylmuramoyl-L-alanine amidase [EC: 3.5.1.28] (inferred from 100% identity to rru:Rru_A2148)

Predicted SEED Role

"N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)" (EC 3.5.1.28)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.28

Use Curated BLAST to search for 3.5.1.28

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RSE7 at UniProt or InterPro

Protein Sequence (522 amino acids)

>Rru_A2148 N-acetylmuramoyl-L-alanine amidase (NCBI) (Rhodospirillum rubrum S1H)
MERYVGRGTTPPMARRKATGVSRRSLLAGTPFVAAALLAPRPALAATRATGLRLGDHGGS
TRFVLELSGPVTWQLVSLSAPYRLIIDMPEVEFALGLVPRDTGLVAGARYGVVRPGFSRM
VIDLTGPAMVDKDFLLEPREGFGWRLVMDLVPTTPEIFMAGVGQPAKPVVPATVTPEPST
RAVAFAAAAAPAAAASGTTAPTPIGPGGPVNLRPAAVGKGPVTVNLASLPSAQAPIADPS
IPGSFGGPGGAAAGEGMQVMAREGPAIVRLADGRRVPIPLEKPQRSVDPVRKPVIVLDPG
HGGKDPGAIGASGTYEKIVTLEMARQLKRALEATGRYKVVLTRESDTSVRLRERIAFGRH
AGADLFVSIHADAMANPQVRGLSVYTLSETASDDEAAQLADRENKVDILLGMDLSQESPD
VATILIDLAQRETKNKSVHFANTLVSALPGDVLKLEKTRRYAGFAVLKAPDVPSVLVEMG
FLSNVSDEKLLRTAPYRAKLAAALVRSVDDFFAPQQQRAWKP