Protein Info for Rru_A1976 in Rhodospirillum rubrum S1H

Annotation: Phenylacetate--CoA ligase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 56% identical to PAAK_AZOEV: Phenylacetate-coenzyme A ligase (paaK) from Azoarcus evansii

KEGG orthology group: K01912, phenylacetate-CoA ligase [EC: 6.2.1.30] (inferred from 100% identity to rru:Rru_A1976)

MetaCyc: 56% identical to phenylacetate-CoA ligase (aerobic) (Aromatoleum evansii)
Phenylacetate--CoA ligase. [EC: 6.2.1.30]

Predicted SEED Role

"Phenylacetate-coenzyme A ligase (EC 6.2.1.30)" in subsystem Aromatic amino acid interconversions with aryl acids (EC 6.2.1.30)

MetaCyc Pathways

penicillin G and penicillin V biosynthesis (1/4 steps found)
phenylacetate degradation II (anaerobic) (1/4 steps found)
phenylacetate degradation I (aerobic) (4/9 steps found)
superpathway of phenylethylamine degradation (4/11 steps found)
L-phenylalanine degradation IV (mammalian, via side chain) (2/9 steps found)
anaerobic aromatic compound degradation (Thauera aromatica) (3/27 steps found)

KEGG Metabolic Maps

Phenylalanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.30

Use Curated BLAST to search for 6.2.1.30

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RSW9 at UniProt or InterPro

Protein Sequence (437 amino acids)

>Rru_A1976 Phenylacetate--CoA ligase (NCBI) (Rhodospirillum rubrum S1H)
MHISNDGYMFAKDRETMPREHLAGLQFARLKDTLTRVYASVPHYRRAFEAKGVSPEDLNT
LEDLARFPFTVKDDLREAYPFKLFAVPRDQVVRLHASSGTTGLPTVVGYTRADIDTWAGL
MARCIACAGGRPGDILHNAYGYGLFTGGLGAHYGAEALGCSVVPMSGGNTEKQILLLKDF
GANVVCCTPSYAINLAEEAERRGVDLKGGPLRVGLFGAEPWCQGMRLELEERLGIRAMDV
YGLSEIMGPGVAVECAEQAGLHGWEDHFLFEVIDPETLRPLPVGEQGELVITTLTKQALP
MIRYRTRDITRLSDEPCPCGRTHRRILRVTGRNDDMMIIRGVNVYPSQLEAVMVGRPGLA
PHYQLILQRDGSLDTLTVEVEAREGLPCDDYDAIGRDLGRHIKSLVGVTARIIVVPPGQI
ARSQGKAARVRDLRKQY