Protein Info for Rru_A1954 in Rhodospirillum rubrum S1H

Annotation: Secretion protein HlyD (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 signal peptide" amino acids 1 to 12 (12 residues), see Phobius details transmembrane" amino acids 13 to 31 (19 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 47 to 238 (192 residues), 98.9 bits, see alignment E=1.4e-32 PF13533: Biotin_lipoyl_2" amino acids 49 to 96 (48 residues), 58.7 bits, see alignment 7.7e-20 PF00529: CusB_dom_1" amino acids 140 to 287 (148 residues), 30.9 bits, see alignment E=4.2e-11 PF16576: HlyD_D23" amino acids 154 to 232 (79 residues), 37.4 bits, see alignment E=3.4e-13 PF13437: HlyD_3" amino acids 157 to 238 (82 residues), 37.1 bits, see alignment E=9e-13

Best Hits

Swiss-Prot: 39% identical to AAEA_YERPS: p-hydroxybenzoic acid efflux pump subunit AaeA (aaeA) from Yersinia pseudotuberculosis serotype I (strain IP32953)

KEGG orthology group: None (inferred from 100% identity to rru:Rru_A1954)

Predicted SEED Role

"possible FusE-MFP/HlyD family membrane fusion protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RSZ1 at UniProt or InterPro

Protein Sequence (300 amino acids)

>Rru_A1954 Secretion protein HlyD (NCBI) (Rhodospirillum rubrum S1H)
MTLKPVLSLLGRYALTLCLVAGATLIAWEAWSRSERTPWTRDGRVSADVVRIAPEVSGTV
SAVPIRDNQYVHRGDILYGIDPERLRLAVAVAEADLEAKRQDMLVRQATARRHGQLRDVV
SQEAVQQAGGAAAVASAAYQAALATLDLAKLDLARATVRSPVDGYVINLRLRPGDYTTAG
VTTVAVLDAARFWITGYFEETKIGQIRVGSPARIRLMGFDRPVSGHVESIGHGIENGNDA
PGPLGLPTVAATFSWVRLAQRIPVRIHVDQVPLDVALAAGMTATIEIIPAAGEPAVGNRR