Protein Info for Rru_A1933 in Rhodospirillum rubrum S1H

Annotation: Uroporphyrin-III C-methyltransferase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 499 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details TIGR01470: siroheme synthase, N-terminal domain" amino acids 4 to 206 (203 residues), 197.7 bits, see alignment E=1.7e-62 PF13241: NAD_binding_7" amino acids 6 to 118 (113 residues), 79.8 bits, see alignment E=3e-26 PF10414: CysG_dimeriser" amino acids 153 to 210 (58 residues), 56.8 bits, see alignment 2.2e-19 TIGR01469: uroporphyrinogen-III C-methyltransferase" amino acids 224 to 465 (242 residues), 258.4 bits, see alignment E=7.1e-81 PF00590: TP_methylase" amino acids 226 to 440 (215 residues), 171.8 bits, see alignment E=3e-54

Best Hits

Swiss-Prot: 46% identical to CYSG_CHRVO: Siroheme synthase (cysG) from Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757)

KEGG orthology group: K02302, uroporphyrin-III C-methyltransferase / precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase [EC: 1.3.1.76 2.1.1.107 4.99.1.4] (inferred from 100% identity to rru:Rru_A1933)

Predicted SEED Role

"Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis or Coenzyme B12 biosynthesis or Dissimilatory nitrite reductase (EC 1.3.1.76, EC 2.1.1.107, EC 4.99.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.107

Use Curated BLAST to search for 1.3.1.76 or 2.1.1.107 or 4.99.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RT12 at UniProt or InterPro

Protein Sequence (499 amino acids)

>Rru_A1933 Uroporphyrin-III C-methyltransferase (NCBI) (Rhodospirillum rubrum S1H)
MDSFPIFLTLTGVRVLVVGGGAAAAAKARLALKAGARVSVFAPALSADWQDLRGHPALDH
RQGFPGQGGEEEWRDVRLVYAAGESLEEDRAVHAAASARGVLVNVVDRPELCDFLTPAIV
DRSPIVVAIGTGGRAPVLARRVRAAIDRLLPARLGALARLAEGFRGTVAQALPDGDRRRR
FWDRFFDGAPALAALEGDEPRARTAALRLLNDGEALDNGAPAAGVVHLVGAGPGDPDLLT
LKAQRLLQEADALVYDDLVGEGILDMARRDAARIYVGKRKGRASLTQEQIGRLMCDLAAA
GRVVVRLKGGDPAVFARLSEEIDALAAAGVAWTVVPGITAASAAAAAIGISLTDRDGAPS
VTLLSGHRRAPAAGESEAAVDWRAAARTGGTLAVYMGLSRATAAAEGLIEGGLSAETPVA
VVHDASRQGQRVSRGTLGELPRLVAQGALAAPDAPGLVLVGKVVAKAGRLPAIAPVFSTP
RAFAGDPSFASQALPSQAG