Protein Info for Rru_A1886 in Rhodospirillum rubrum S1H

Annotation: 2-dehydro-3-deoxyphosphooctonate aldolase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 280 transmembrane" amino acids 38 to 54 (17 residues), see Phobius details PF00793: DAHP_synth_1" amino acids 11 to 269 (259 residues), 247.6 bits, see alignment E=5.7e-78 TIGR01362: 3-deoxy-8-phosphooctulonate synthase" amino acids 18 to 274 (257 residues), 396.9 bits, see alignment E=1.6e-123

Best Hits

Swiss-Prot: 71% identical to KDSA_RHOPS: 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) from Rhodopseudomonas palustris (strain BisB5)

KEGG orthology group: K01627, 2-dehydro-3-deoxyphosphooctonate aldolase (KDO 8-P synthase) [EC: 2.5.1.55] (inferred from 100% identity to rru:Rru_A1886)

MetaCyc: 44% identical to 3-deoxy-D-manno-octulosonate 8-phosphate synthase (Escherichia coli K-12 substr. MG1655)
3-deoxy-8-phosphooctulonate synthase. [EC: 2.5.1.55]

Predicted SEED Role

"2-Keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase (EC 2.5.1.55)" in subsystem KDO2-Lipid A biosynthesis (EC 2.5.1.55)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.55

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RT59 at UniProt or InterPro

Protein Sequence (280 amino acids)

>Rru_A1886 2-dehydro-3-deoxyphosphooctonate aldolase (NCBI) (Rhodospirillum rubrum S1H)
MTPRHVAVGPVTFGNDLPMVLIAGPCQMESREHALETASILVALCADLGVPFIYKTSFDK
ANRTSLSGQRGVGMEKALPIFAEIRETLGCPVLTDVHDAGQCAPVAEAVDVLQIPAFLCR
QTDLLLAAAATGRVINVKKGQFLAPWDMKNVANKVMSAGNERVLLTERGASFGYNTLVTD
MRSLPIMARDTGCPIIIDATHSVQQPGGQGGSSGGQREFAPVIARAALAVGVAGVFLETH
ADPDHAPSDGPNMIALDAMPALIAVLRDLDAFAKKYPVTL